Single Domain Antibodies [E-Book]: Methods and Protocols / edited by Dirk Saerens, Serge Muyldermans.
Muyldermans, Serge.
Saerens, Dirk.
Totowa, NJ : Humana Press, 2012
XV, 580 p. 89 illus., 35 illus. in color. digital.
englisch
9781617799686
10.1007/978-1-61779-968-6
Methods in molecular biology, methods and protocols ; 911
Full Text
Table of Contents:
  • From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small
  • Introduction to Heavy Chain Antibodies and Derived Nanobodies
  • Overview and Discovery of IgNARs and Generation of VNARs
  • Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and Vκ Domain Antibodies
  • Preparation of a Naïve Library of Camelid Single Domain Antibodies
  • Selection by Phage Display of Single Domain Antibodies Specific to Antigens in Their Native Conformation
  • Semi-Automated Panning of Naive Camelidae Libraries and Selection of Single-Domain Antibodies Against Peptide Antigens
  • Pichia Surface Display: A Tool for Screening Single Domain Antibodies
  • Bacterial Two Hybrid: A Versatile One-Step Intracellular Selection Method
  • Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies
  • Selection of Functional Single Domain Antibody Fragments for Interfering with Protein-Protein Interactions Inside Cells: A “One Plasmid” Mammalian Two-Hybrid System
  • Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization
  • Selection of VHHs Under Application Conditions
  • Isolation and Characterization of Clostridium difficile Toxin-Specific Single-Domain Antibodies
  • Selection of VHH Antibody Fragments that Recognize Different Aβ Depositions Using Complex Immune Libraries
  • Expression of Single-Domain Antibodies in Bacterial Systems
  • Expression of VHHs in S. cerevisiae
  • Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells
  • Production of Camel-Like Antibodies in Plants
  • Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains
  • Solubility and Stability Engineering of Human VH Domains
  • Improvement of Proteolytic Stability through In Silico Engineering
  • Selection of Human VH Single Domains with Improved Biophysical Properties by Phage Display
  • Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction
  • Characterization of Single-Domain Antibodies with an Engineered Disulfide Bond
  • Affinity Maturation of Single-Domain Antibodies by Yeast Surface Display
  • Multivalent Display of Single-Domain Antibodies
  • Methods for Determining the PK Parameters of AlbudAbs and of Long Serum Half Life Drugs Made Using the AlbudAb Technology
  • Fluorescent Protein Specific Nanotraps to Study Protein-Protein Interactions and Histone-Tail Peptide Binding
  • Site-Specific Labeling of His-Tagged Nanobodies with 99mTc: A Practical Guide
  • Nanobody-Based Chromatin Immunoprecipitation
  • User-Friendly Expression Plasmids Enable the Fusion of VHHs to Application-Specific Tags
  • Application of Single Domain Antibodies in Tumor Histochemistry
  • Nanobodies as Structural Probes of Protein Misfolding and Fibril Formation
  • Molecular Imaging Using Nanobodies: A Case Study
  • Case Study on Live Cell Apoptosis-Assay Using Lamin-Chromobody Cell-Lines for High-Content Analysis..