Antibody Engineering [E-Book]: Methods and Protocols, Second Edition / edited by Patrick Chames.
More than ever, antibodies are being recognized as a major drug modality in a variety of diseases, including cancer, autoimmune diseases, infectious diseases, or even neurodegenerative disorders. Over 30 therapeutic antibodies have been approved and novel molecules are entering clinical trials at an...
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Full text |
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Personal Name(s): | Chames, Patrick. |
Edition: |
2nd ed. 2012. |
Imprint: |
Totowa, NJ :
Humana Press,
2012
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Physical Description: |
XVI, 734 p. 98 illus., 41 illus. in color. digital. |
Note: |
englisch |
ISBN: |
9781617799747 |
DOI: |
10.1007/978-1-61779-974-7 |
Series Title: |
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Methods in molecular biology, methods and protocols ;
907 |
Subject (LOC): |
- Use of IMGT® Databases and Tools for Antibody Engineering and Humanization
- Computer-Assisted Modeling of Antibody Variable Domains
- Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells
- Human in Cell scFv Library from Infiltrating B Cell
- Construction of Human Naive Antibody Gene Libraries
- Synthetic Customized scFv Libraries
- Selection of Stable scFv Antibodies by Phage Display
- Generation of Single Domain Antibody Fragments Derived from Camelids and Generation of Manifold Constructs
- Generation and Isolation of Target Specific Single Domain Antibodies from Shark Immune Repertoires.-Generation of Human Single Domain Antibody Repertoires by Kunkel Mutagenesis
- Phage Display and Selections on Purified Antigens
- Phage Display and Selections on Cells
- Humanization by CDR Grafting and Specificity-Determining Residue Grafting.-Humanization by Guided Selections
- Selection of Antibody Fragments by Yeast Display
- Evolution of Antibodies in vitro by Ribosome Display
- Mammalian Cell Display of Full Length IgG
- Production of Antibody Fragments in Escherichia coli.-Production of Antibody Derivatives in the Methylotrophic Yeast Pichia pastoris
- Monoclonal Antibody Expression in Mammalian Cells
- Production of Recombinant Antibodies in Drosophila melanogaster S2 Cells
- Production of Antibody Fragments Using the Baculovirus-Insect Cell System
- Transient and Stable Expression of Antibodies in Nicotiana Species
- Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance
- Affinity Determination of Biotinylated Antibodies by Flow Cytometry
- Affinity Maturation of Antibodies: Optimized Methods to Generate High Quality scFv Libraries and Isolate IgG Candidates by High Throughput Screening
- Affinity Maturation by Semi Rational Approaches
- Molecular Scanning Mutagenesis: Combining Ribosome Display, Random Mutagenesis and Bioinformatic Analysis for Protein Engineering
- Fucose-Targeted Glyco-Engineering of Pharmaceutical Cell Lines
- Fc engineering: Design, expression and functional characterization of antibody variants with improved effector function
- Fc engineering: Serum half life modulation through FcRn binding
- Monoclonal Antibody Lead Characterization - In vitro and in vivo methods
- Production, generation and characterization of scFv TNF ligand fusion proteins
- Antibody-IL-2 fusion proteins for tumor targeting
- Recombinant immunotoxins containing low endotoxins for clinical and animal studies
- Chimeric antigen receptors for T-cell based therapy
- Selection and Use of Intracellular Antibodies (Intrabodies)
- Radiolabeled Antibodies for Imaging and Therapy
- Design and Production of Multimeric Antibody Fragments, Focused on Diabodies with Enhanced Clinical Efficacy
- Production of Bispecific Antibodies: Diabodies and Tandem scFv..