Antibody Engineering [E-Book]: Methods and Protocols, Second Edition / edited by Patrick Chames.
Chames, Patrick.
2nd ed. 2012.
Totowa, NJ : Humana Press, 2012
XVI, 734 p. 98 illus., 41 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 907
Full Text
Table of Contents:
  • Use of IMGT® Databases and Tools for Antibody Engineering and Humanization
  • Computer-Assisted Modeling of Antibody Variable Domains
  • Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells
  • Human in Cell scFv Library from Infiltrating B Cell
  • Construction of Human Naive Antibody Gene Libraries
  • Synthetic Customized scFv Libraries
  • Selection of Stable scFv Antibodies by Phage Display
  • Generation of Single Domain Antibody Fragments Derived from Camelids and Generation of Manifold Constructs
  • Generation and Isolation of Target Specific Single Domain Antibodies from Shark Immune Repertoires.-Generation of Human Single Domain Antibody Repertoires by Kunkel Mutagenesis
  • Phage Display and Selections on Purified Antigens
  • Phage Display and Selections on Cells
  • Humanization by CDR Grafting and Specificity-Determining Residue Grafting.-Humanization by Guided Selections
  • Selection of Antibody Fragments by Yeast Display
  • Evolution of Antibodies in vitro by Ribosome Display
  • Mammalian Cell Display of Full Length IgG
  • Production of Antibody Fragments in Escherichia coli.-Production of Antibody Derivatives in the Methylotrophic Yeast Pichia pastoris
  • Monoclonal Antibody Expression in Mammalian Cells
  • Production of Recombinant Antibodies in Drosophila melanogaster S2 Cells
  • Production of Antibody Fragments Using the Baculovirus-Insect Cell System
  • Transient and Stable Expression of Antibodies in Nicotiana Species
  • Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance
  • Affinity Determination of Biotinylated Antibodies by Flow Cytometry
  • Affinity Maturation of Antibodies: Optimized Methods to Generate High Quality scFv Libraries and Isolate IgG Candidates by High Throughput Screening
  • Affinity Maturation by Semi Rational Approaches
  • Molecular Scanning Mutagenesis: Combining Ribosome Display, Random Mutagenesis and Bioinformatic Analysis for Protein Engineering
  • Fucose-Targeted Glyco-Engineering of Pharmaceutical Cell Lines
  • Fc engineering: Design, expression and functional characterization of antibody variants with improved effector function
  • Fc engineering: Serum half life modulation through FcRn binding
  • Monoclonal Antibody Lead Characterization - In vitro and in vivo methods
  • Production, generation and characterization of scFv TNF ligand fusion proteins
  • Antibody-IL-2 fusion proteins for tumor targeting
  • Recombinant immunotoxins containing low endotoxins for clinical and animal studies
  • Chimeric antigen receptors for T-cell based therapy
  • Selection and Use of Intracellular Antibodies (Intrabodies)
  • Radiolabeled Antibodies for Imaging and Therapy
  • Design and Production of Multimeric Antibody Fragments, Focused on Diabodies with Enhanced Clinical Efficacy
  • Production of Bispecific Antibodies: Diabodies and Tandem scFv..