Plant Salt Tolerance [E-Book]: Methods and Protocols / edited by Sergey Shabala, Tracey Ann Cuin.
Cuin, Tracey Ann.
Shabala, Sergey.
Totowa, NJ : Humana Press, 2012
XIV, 432 p. 56 illus., 26 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 913
Full Text
Table of Contents:
  • Patch-Clamp Protocols to Study Cell Ionic Homeostasis under Saline Conditions
  • Studying Plant Salt Tolerance with the Voltage Clamp Technique
  • Using the Multifunctional Xylem Pprobe for In situ Studies of Plant Water and Ion Relations under Saline Conditions
  • Measuring Intracellular Ion Concentrations with Multi-Barrelled Microelectrodes
  • Single-Cell Sampling and Analysis (SiCSA)
  • Non-Invasive Flux Measurements Using Microsensors: Theory, Limitations and Systems
  • Quantifying Kinetics of Net Ion Fluxes from Plant Tissues by Non-Invasive Microelectrode Measuring MIFE Technique
  • Quantitative Cryo-Analytical Scanning Electron Microscopy (CEDX): An Important Technique Useful for Cell-Specific Localization of Salt
  • Fluorescence Lifetime Imaging (FLIM) Measurements in Salinity Research
  • Cytosolic Ca2+ Determinations in Studying Plant Responses to Salinity and Oxidative Stress
  • Infrared Thermography in Plant Phenotyping for Salinity Tolerance
  • In vivo Imaging of Nitric Oxide and Reactive Oxygen Species Using Laser Scanning Confocal Microscopy
  • Metabolomics for Salinity Research.-Purification of Plant Plasma Membranes by Two-Phase Partitioning and Measurement of H+ Pumping.-Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues.-Quantification of the Antioxidant Activity in Salt-Stressed Tissues.-Quantification of Abscisic Acid, Cytokinin and Auxin Content in Salt-Stressed Plant Tissues.-Fluorescence-Activated Cell Sorting for Analysis of Cell Type-Sspecific Responses to Salinity Stress in Arabidopsis and Rice.-Transformation Using Controlled cDNA Overexpression System.-Transcriptome Analysis of Membrane Transporters in Response to Salinity Stress.-Marker-Assisted Selection in Plant Breeding for Salinity Tolerance.-Transcriptomics on Small Samples.-Plastid Transformation for Abiotic Stress Tolerance in Plants.-Manipulating Expression of Tonoplast Transporters.-Using Heterologous Expression Systems to Characterize Potassium and Sodium Transport Activities.-Isotope Techniques to Study Kinetics of Na+ and K+ Transport Under Salinity Conditions.-Trait Dissection of Salinity Tolerance with Plant Phenomics.-Measuring Soil Salinity..