Genomic Imprinting [E-Book]: Methods and Protocols / edited by Nora Engel.
Engel, Nora.
Totowa, NJ : Humana Press, 2012
XI, 297 p. 40 illus., 19 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 925
Full Text
Table of Contents:
  • Uniparental Embryos in the Study of Genomic Imprinting
  • Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Species
  • Generation of Trophoblast Stem Cells
  • Immunomagnetic Purification of Murine Primordial Germ Cells
  • Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA
  • Identification of Imprinted Loci by Transcriptome Sequencing
  • Data Mining as a Discovery Tool for Imprinted Genes
  • Engineering of Large Deletions and Duplications In Vivo
  • Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells
  • Chromatin Immunoprecipitation to Characterize the Epigenetic Profiles of Imprinted Domains
  • Quantitative Chromosome Conformation Capture
  • Genome-Wide Analysis of DNA Methylation in Low Cell Numbers by Reduced Representation Bisulfite Sequencing
  • Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Studies
  • Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs
  • Co-Immunoprecipitation of Long Non-Coding RNAs
  • Specialized Technologies for Epigenetics in Plants
  • Computational Studies of Imprinted Genes
  • Insights on Imprinting from Beyond Mice and Men
  • Non-Mammalian Parent-Of-Origin Effects..