Single-Stranded DNA Binding Proteins [E-Book]: Methods and Protocols / edited by James L. Keck.
Keck, James L.
Totowa, NJ : Humana Press, 2012
X, 259 p. 40 illus., 20 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 922
Full Text
Table of Contents:
  • Functions of Single-Strand DNA-Binding Proteins in DNA Replication, Recombination and Repair.-Structural Diversity Based on Variability in Quaternary Association. A Case Study Involving Eubacterial and Related SSBs
  • SB Binding to ssDNA Using Isothermal Titration Calorimetry
  • SSB-DNA Binding Monitored by Fluorescence Intensity and Anisotropy
  • Single Molecule Analysis of SSB Dynamics on Single Stranded DNA
  • Sample Preparation Methods to Analyze DNA-induced Structural Changes in Replication Protein A (RPA)
  • Structural Studies of SSB Interaction with RecO
  • Investigation of Protein-Protein Interactions of Single-Stranded DNA-Binding Proteins by Analytical Ultracentrifugation
  • Ammonium Sulfate Co-Precipitation of SSB and Interacting Proteins
  • Analyzing Interactions Between SSB and Proteins by the Use of Fluorescence Anisotropy
  • Far Western Blotting as a Rapid and Efficient Method for Detecting Interactions Between DNA Replication and DNA Repair Proteins
  • Methods for Analysis of SSB/Protein Interactions by SPR
  • Use of Native Gels to Measure Protein Binding to SSB
  • Identification of Small Molecules That Disrupt SSB/Protein Interactions Using a High-Throughput Screen
  • Detection of Post-translational Modifications of Replication Protein A
  • Detecting Posttranslational Modification of Bacterial SSB Proteins
  • Fluorescent SSB as a Reagentless Biosensor for Single-Stranded DNA
  • Fluorescent Single-Stranded DNA-Binding Proteins Enable in vitro and in vivo Studies
  • Use of Fluorescently Tagged SSB Proteins in in vivo Localization Experiments..