Spermatogenesis [E-Book]: Methods and Protocols / edited by Douglas T. Carrell, Kenneth I. Aston.
Aston, Kenneth I.
Carrell, Douglas T.
Totowa, NJ : Humana Press, 2013
XVII, 554 p. 110 illus., 68 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 927
Full Text
Table of Contents:
  • Methods For Sperm Concentration Determination
  • Methods of Sperm Vitality Assessment
  • The Hypo-osmotic Swelling Test for Evaluation of Sperm Membrane Integrity
  • Sperm Morphology Classification: A Rational Method For Schemes Adopted By The World Health Organization
  • Sperm Morphology Assessment Using Strict (Tygerberg) Criteria
  • Methods For Direct And Indirect Antisperm Antibody Testing
  • Manual Methods For Sperm Motility Assessment
  • Computer-Aided Sperm Analysis (CASA) Of Sperm Motility And Hyperactivation
  • The Hemizona Assay For Assessment Of Sperm Function
  • The Sperm Penetration Assay for the Assessment of Fertilization Capacity
  • Methods For The Assessment Of Sperm Capacitation And Acrosome Reaction Excluding The Sperm Penetration Assay
  • Sperm DNA Fragmentation Analysis Using the TUNEL Assay
  • Sperm DNA Damage Measured by Comet Assay
  • Sperm Chromatin Structure Assay (SCSA®)
  • Sperm Aneuploidy Testing Using Fluorescence In Situ Hybridization (FISH)
  • Flow Cytometric Methods For Sperm Assessment
  • Human Y Chromosome Microdeletion Analysis By PCR Multiplex Protocols Identifying Only Clinically Relevant AZF Microdeletions
  • Sperm Cryopreservation Methods
  • Density Gradient Separation Of Sperm For Artificial Insemination
  • Recovery, Isolation, Identification and Preparation of Spermatozoa from Human Testis
  • Enhancement of Sperm Motility Using Pentoxifylline and Platelet-Activating Factor
  • Intracytoplasmic Morphology Selected Sperm Injection
  • Sperm Selection For ICSI Using Annexin V
  • Sperm Selection for ICSI Using the Hyaluronic Acid Binding Assay
  • Sperm Selection Based On Electrostatic Charge
  • Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting
  • Assessment of Spermatogenesis Through Staging of Seminiferous Tubules
  • Immunohistochemical Approaches for the Study of Spermatogenesis
  • Ultrastructural Analysis of Testicular Tissue and Semen by Transmission and Scanning Electron Microscopy
  • Assessment Of Oxidative Stress In Sperm And Semen
  • Improved Chemiluminescence Assay for Measuring Antioxidant Capacity of Seminal Plasma
  • Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies
  • Isolating mRNA and Small Noncoding RNAs From Human Sperm
  • A Review Of Genome-Wide Approaches To Study The Genetic Basis For Spermatogenic Defects
  • Methods For The Analysis Of The Sperm Proteome
  • Methodology of Aniline Blue Staining Of Chromatin and the Assessment of the Associated Nuclear and Cytoplasmic Attributes in Human Sperm
  • Isolation of Sperm Nuclei and Nuclear Matrices from the Mouse, and Other Rodents
  • Extraction and Analysis of Human Sperm Protamine 1 / Protamine 2 Ratio Using Acid Gel Electrophoresis
  • Analysis of Gene-Specific and Genome-Wide Sperm DNA Methylation
  • Evaluating the Localization and DNA Binding Complexity of Histones in Mature Sperm
  • In Vitro Spermatogenesis Using An Organ Culture Technique
  • Testicular Tissue Grafting and Male Germ Cell Transplantation
  • Transgenic Modification of Spermatogonial Stem Cells Using Lentiviral Vectors
  • Methods for Sperm-Mediated Gene Transfer
  • Phenotypic Assessment of Male Fertility Status in Transgenic Animal Models..