Recombinant and In Vitro RNA Synthesis [E-Book]: Methods and Protocols / edited by Graeme L. Conn.
Conn, Graeme L.
Totowa, NJ : Humana Press, 2012
XIV, 274 p. 56 illus. digital.
englisch
9781627031134
10.1007/978-1-62703-113-4
Methods in molecular biology, methods and protocols ; 941
Full Text
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245 1 0 |a Recombinant and In Vitro RNA Synthesis  |h [E-Book]:  |b Methods and Protocols /  |c edited by Graeme L. Conn. 
264 1 |a Totowa, NJ :  |b Humana Press,  |c 2012  |e (Springer LINK) 
300 |a XIV, 274 p. 56 illus.  |b digital. 
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490 |a Methods in molecular biology, methods and protocols ;   |v 941 
500 |a englisch 
505 0 |a Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold.-Selective RNase H Cleavage of Target RNAs  from a tRNA Scaffold -- Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR -- General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE -- Preparation of Short RNA by in vitro Transcription -- Native RNA Purification by Gel Filtration Chromatography -- Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5’ and 3’ Ends -- Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends -- Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods.-Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA -- Affinity Purification of RNA using an ARiBo Tag -- Plasmid Template Design and in vitro Transcription of Short RNAs within a ‘Structure Cassette’ for Structure Probing Experiments -- In Vitro Transcription of Modified RNAs -- End-Labeling Oligonucleotides with Chemical Tags After Synthesis -- High-Purity Enzymatic Synthesis of  Site-Specifically Modified tRNA -- Se-derivatized RNAs for X-ray Crystallography -- Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs -- Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC -- Splint Ligation of RNA with T4 DNA Ligase.. 
520 |a The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the field detail approaches from the inception of a new RNA project to the final sample ready for analysis. These experimental protocols combine established work-horse approaches with some of the latest innovative methods for RNA synthesis and purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant and In Vitro RNA Synthesis: Methods and Protocols aids scientists in continuing to study the fascinating world of RNA. 
650 0 |a Human genetics. 
650 0 |a Medicine. 
650 0 |a Nucleic acids. 
700 1 |a Conn, Graeme L. 
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