siRNA Design [E-Book]: Methods and Protocols / edited by Debra J. Taxman.
Taxman, Debra J.
Totowa, NJ : Humana Press, 2013
XIII, 392 p. 66 illus., 40 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 942
Full Text
Table of Contents:
  • What Parameters to Consider and Which Software Tools to Use for Target Selection and Molecular Design of Small Interfering RNAs
  • Methods for Selecting Effective siRNA Target Sequences Using a Variety of Statistical and Analytical Techniques
  • Designing Functional siRNA with Reduced Off-Target Effects
  • Design and Screening of siRNAs Against Highly Structured RNA Targets
  • Engineering Small Interfering RNAs by Strategic Chemical Modification
  • The Design, Selection and Evaluation of Highly Specific and Functional siRNA Incorporating Unlocked Nucleobase Analogs
  • The Design, Preparation, and Evaluation of Asymmetric Small Interfering RNA for Specific Gene Silencing in Mammalian Cells
  • Design of Nuclease-Resistant Fork-Like Small Interfering RNA (fsiRNA)
  • Designing Dual-Targeting siRNA Duplexes Having Two Active Strands that Combine siRNA and MicroRNA-Like Targeting
  • Strategies for Designing and Validating Immunostimulatory siRNAs
  • Designing Efficient and Specific Endoribonuclease Prepared siRNAs (esiRNAs)
  • Short Hairpin RNA-Mediated Gene Silencing
  • Design of Lentivirally Expressed siRNAs
  • Bi-Functional Short Hairpin RNA (bi-shRNA): Design and Pathway to Clinical Application
  • Design and Chemical Modification of Synthetic Short shRNAs as Potent RNAi Triggers
  • Production and Application of Long dsRNA in Mammalian Cells
  • Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors
  • Construction of shRNA Expression Plasmids for Silkworm Cell Lines Using Single-Stranded DNA and Bst DNA Polymerase
  • Designing Effective amiRNA and Multimeric amiRNA Against Plant Viruses
  • Downregulation of Plant Genes with MiRNA Induced Gene Silencing..