siRNA Design [E-Book]: Methods and Protocols / edited by Debra J. Taxman.
The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: Methods and Protocols offers...
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Full text |
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Personal Name(s): | Taxman, Debra J. |
Imprint: |
Totowa, NJ :
Humana Press,
2013
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Physical Description: |
XIII, 392 p. 66 illus., 40 illus. in color. digital. |
Note: |
englisch |
ISBN: |
9781627031196 |
DOI: |
10.1007/978-1-62703-119-6 |
Series Title: |
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Methods in molecular biology, methods and protocols ;
942 |
Subject (LOC): |
- What Parameters to Consider and Which Software Tools to Use for Target Selection and Molecular Design of Small Interfering RNAs
- Methods for Selecting Effective siRNA Target Sequences Using a Variety of Statistical and Analytical Techniques
- Designing Functional siRNA with Reduced Off-Target Effects
- Design and Screening of siRNAs Against Highly Structured RNA Targets
- Engineering Small Interfering RNAs by Strategic Chemical Modification
- The Design, Selection and Evaluation of Highly Specific and Functional siRNA Incorporating Unlocked Nucleobase Analogs
- The Design, Preparation, and Evaluation of Asymmetric Small Interfering RNA for Specific Gene Silencing in Mammalian Cells
- Design of Nuclease-Resistant Fork-Like Small Interfering RNA (fsiRNA)
- Designing Dual-Targeting siRNA Duplexes Having Two Active Strands that Combine siRNA and MicroRNA-Like Targeting
- Strategies for Designing and Validating Immunostimulatory siRNAs
- Designing Efficient and Specific Endoribonuclease Prepared siRNAs (esiRNAs)
- Short Hairpin RNA-Mediated Gene Silencing
- Design of Lentivirally Expressed siRNAs
- Bi-Functional Short Hairpin RNA (bi-shRNA): Design and Pathway to Clinical Application
- Design and Chemical Modification of Synthetic Short shRNAs as Potent RNAi Triggers
- Production and Application of Long dsRNA in Mammalian Cells
- Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors
- Construction of shRNA Expression Plasmids for Silkworm Cell Lines Using Single-Stranded DNA and Bst DNA Polymerase
- Designing Effective amiRNA and Multimeric amiRNA Against Plant Viruses
- Downregulation of Plant Genes with MiRNA Induced Gene Silencing..