Fungal Secondary Metabolism [E-Book]: Methods and Protocols / edited by Nancy P. Keller, Geoffrey Turner.
Keller, Nancy P.
Turner, Geoffrey.
Totowa, NJ : Humana Press, 2012
XII, 288 p. 60 illus., 32 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 944
Full Text
Table of Contents:
  • Library Preparation and Data Analysis Packages for Rapid Genome Sequencing
  • Bioinformatics Approaches and Software for Detection of Secondary Metabolic Gene Clusters
  • Media and Growth Conditions for Induction of Secondary Metabolite Production
  • Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays
  • The “FERMEX” Method for Metabolite-Enriched Fungal Extracts
  • The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites
  • Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri
  • Production, Detection, and Purification of Clavine-Type Ergot Alkaloids
  • Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS)
  • Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans
  • Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis
  • Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host
  • Multi-Copy Genetic Screen in Aspergillus nidulans
  • Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification
  • Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra
  • Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi
  • Targeted Proteomics for Metabolic Pathway Optimization
  • Investigation of In Vivo Protein Interactions in Aspergillus Spores
  • Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus
  • Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi
  • Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects..