Legionella [E-Book] : Methods and Protocols / edited by Carmen Buchrieser, Hubert Hilbi.
Buchrieser, Carmen.
Hilbi, Hubert.
Totowa, NJ : Humana Press, 2013
XXVIII, 622 p. 86 illus., 35 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 954
Full Text
Table of Contents:
  • Identification of Legionella in the Environment
  • Identification of Legionella in Clinical Samples
  • Sequence-Based Identification of Legionella
  • Investigation of Outbreaks - Epidemiology
  • Outbreak Investigations and Identification of Legionella in Contaminated Water
  • Typing Methods for Legionella
  • Culture, Media and Handling of Legionella
  • The Intracellular Metabolism of Legionella by Isotopologue Profiling
  • Induction of Competence for Natural Transformation in Legionella pneumophila and Exploitation for Mutant Construction
  • Constructing Unmarked Gene Deletions in Legionella pneumophila
  • Natural Biofilm Formation with Legionella pneumophila
  • Production of Legionella pneumophila Monospecies Biofilms in a Low-Nutrient Concentration Medium
  • Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila
  • Analysis of Legionella Infection by Flow Cytometry
  • Analysis of Legionella Infection Using RNAi in Drosophila Cells
  • Immunofluorescence Imaging of Legionella Infection In vitro
  • Electron Microscopy of Legionella and Legionella-Infected Cells
  • Pathogen Vacuole Purification from Legionella-Infected Amoeba and Macrophages
  • Methods for Determining Protein Translocation by the Legionella pneumophila Dot/Icm Type IV Secretion System
  • Subcellular Localization of Legionella Dot/Icm Effectors
  • Purification and Characterization of Legionella U-Box-Type E3 ubiquitin ligase
  • Legionella Phospholipases Implicated in Infection – Determination of Enzymatic Activities
  • Interactions of Legionella Effector Proteins with Host Phosphoinositide Lipids
  • Characterization of Legionella lipopolysaccharide
  • The Tetrahymena and Acanthamoeba Model Systems
  • Dictyostelium Host Response to Legionella infection: Strategies and Assays
  • The Caenorhabditis elegans Model of Legionella Infection
  • Infection of Nonphagocytic Host Cells by Legionella
  • Analyzing Caspase-1 Activation During Legionella pneumophila Infection in Macrophages
  • The Mouse as a Model for Pulmonary Legionella Infection
  • Assessment of Legionella-Specific Immunity in Mice
  • The Guinea Pig Model of Legionnaires’ Disease
  • Human Susceptibility to Legionnaires´ Disease
  • cDNA library Construction for Next Generation Sequencing (NGS) to Determine the Transcriptional Landscape of Legionella pneumophila
  • Methods to Study Legionella Transcriptome in vitro and in vivo
  • Co-Immunoprecipitation: Protein-RNA and Protein-DNA Interaction
  • Identification of Legionella Effectors Using Bioinformatic Approaches. .