Electron Crystallography of Soluble and Membrane Proteins [E-Book] : Methods and Protocols / edited by Ingeborg Schmidt-Krey, Yifan Cheng.
Cheng, Yifan.
Schmidt-Krey, Ingeborg.
Totowa, NJ : Humana Press, 2013
XIII, 586 p. 151 illus., 63 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 955
Full Text
Table of Contents:
  • Introduction to Electron Crystallography
  • Practical Aspects in Expression and Purification of Membrane Proteins for Structural Analysis
  • Two-Dimensional Crystallization of Membrane Proteins by Reconstitution through Dialysis
  • Monolayer Two-Dimensional Crystallization
  • Screening for Two-Dimensional Crystals by Transmission Electron Microscopy of Negatively Stained Samples
  • Low Dose Techniques and Cryo-Electron Microscopy
  • Grid Preparation for Cryo-Electron Microscopy
  • Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins
  • The Collection of High-Resolution Electron Diffraction Data
  • Image Processing of 2D Crystal Images
  • Merging of Image Data in Electron Crystallography
  • Evaluation of Electron Crystallographic Data From Images of Two-Dimensional Crystals
  • Modeling, Docking, and Fitting of Atomic Structures to 3D Maps from Cryo-Electron Microscopy
  • Phasing Electron Diffraction Data by Molecular Replacement: Strategy for Structure Determination and Refinement
  • High-Throughput Methods for Electron Crystallography
  • Automated Grid Handling and Image Acquisition for Two-Dimensional Crystal Screening
  • Automation of Data Acquisition in Electron Crystallography
  • Automation of Image Processing in Electron Crystallography
  • Choice and Maintenance of Equipment for Electron Crystallography
  • Future Developments in Instrumentation for Electron Crystallography
  • Tubular Crystals and Helical Arrays: Structural Determination of HIV-1 Capsid Assemblies Using Iterative Helical Real-Space Reconstruction
  • Single Particle Electron Microscopy
  • Electron Tomography of Paracrystalline 2D Arrays
  • High-Resolution Imaging of 2D Outer Membrane Protein F (OmpF) Crystals by Atomic Force Microscopy
  • Determination of Soluble and Membrane Protein Structures by X-Ray Crystallography
  • Solution Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Structure-Function Insights of Membrane and Soluble Proteins Revealed by Electron Crystallography
  • Lipid Monolayer and Sparse Matrix Screening for Growing Two-Dimensional Crystals for Electron Crystallography: Methods and Examples
  • Processing of Electron Diffraction Patterns with the XDP Program
  • Future Directions of Electron Crystallography. .