Electron Crystallography of Soluble and Membrane Proteins [E-Book] : Methods and Protocols / edited by Ingeborg Schmidt-Krey, Yifan Cheng.
The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins. This technology is very well devel...
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Full text |
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Personal Name(s): | Cheng, Yifan. |
Schmidt-Krey, Ingeborg. | |
Imprint: |
Totowa, NJ :
Humana Press,
2013
|
Physical Description: |
XIII, 586 p. 151 illus., 63 illus. in color. digital. |
Note: |
englisch |
ISBN: |
9781627031769 |
DOI: |
10.1007/978-1-62703-176-9 |
Series Title: |
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Methods in molecular biology, methods and protocols ;
955 |
Subject (LOC): |
- Introduction to Electron Crystallography
- Practical Aspects in Expression and Purification of Membrane Proteins for Structural Analysis
- Two-Dimensional Crystallization of Membrane Proteins by Reconstitution through Dialysis
- Monolayer Two-Dimensional Crystallization
- Screening for Two-Dimensional Crystals by Transmission Electron Microscopy of Negatively Stained Samples
- Low Dose Techniques and Cryo-Electron Microscopy
- Grid Preparation for Cryo-Electron Microscopy
- Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins
- The Collection of High-Resolution Electron Diffraction Data
- Image Processing of 2D Crystal Images
- Merging of Image Data in Electron Crystallography
- Evaluation of Electron Crystallographic Data From Images of Two-Dimensional Crystals
- Modeling, Docking, and Fitting of Atomic Structures to 3D Maps from Cryo-Electron Microscopy
- Phasing Electron Diffraction Data by Molecular Replacement: Strategy for Structure Determination and Refinement
- High-Throughput Methods for Electron Crystallography
- Automated Grid Handling and Image Acquisition for Two-Dimensional Crystal Screening
- Automation of Data Acquisition in Electron Crystallography
- Automation of Image Processing in Electron Crystallography
- Choice and Maintenance of Equipment for Electron Crystallography
- Future Developments in Instrumentation for Electron Crystallography
- Tubular Crystals and Helical Arrays: Structural Determination of HIV-1 Capsid Assemblies Using Iterative Helical Real-Space Reconstruction
- Single Particle Electron Microscopy
- Electron Tomography of Paracrystalline 2D Arrays
- High-Resolution Imaging of 2D Outer Membrane Protein F (OmpF) Crystals by Atomic Force Microscopy
- Determination of Soluble and Membrane Protein Structures by X-Ray Crystallography
- Solution Nuclear Magnetic Resonance (NMR) Spectroscopy
- Structure-Function Insights of Membrane and Soluble Proteins Revealed by Electron Crystallography
- Lipid Monolayer and Sparse Matrix Screening for Growing Two-Dimensional Crystals for Electron Crystallography: Methods and Examples
- Processing of Electron Diffraction Patterns with the XDP Program
- Future Directions of Electron Crystallography. .