Mammalian Oocyte Regulation [E-Book] : Methods and Protocols / edited by Hayden A. Homer.
Homer, Hayden A.
Totowa, NJ : Humana Press, 2013
XI, 311 p. 61 illus., 19 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 957
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Table of Contents:
  • Studying Recombination in Mouse Oocytes
  • Analysis of Recombinants in Female Mouse Meiosis
  • Studying Meiosis Specific Cohesins in Mouse Embryonic Oocytes
  • Immunohistochemical Approaches to the Study of Human Fetal Ovarian Development
  • Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos
  • Time-Lapse Epi-Fluorescence Imaging of Expressed cRNA to Cyclin B1 for Studying Meiosis I in Mouse Oocytes
  • Using FRET to Study RanGTP Gradients in Live Mouse Oocytes
  • Making cRNA for Microinjection and Expression of Fluorescently-Tagged Proteins for Live-Cell Imaging in Oocytes
  • RNAi-Based Methods for Gene Silencing in Mouse Oocytes
  • Micro-Injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes
  • Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos
  • Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes
  • Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
  • Chromosome Spreads with Centromere Staining in Mouse Oocytes
  • Preparation of Mammalian Oocytes for Transmission Electron Microscopy
  • Measuring Ca2+ Oscillations in Mammalian Eggs
  • Counting Chromosomes in Intact Eggs
  • Free-Hand Bisection of Mouse Oocytes and Embryos
  • Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes
  • Nuclear Transfer in the Mouse Oocyte. .