p53 Protocols [E-Book] / edited by Sumitra Deb, Swati Palit Deb.
Deb, Sumitra.
Deb, Swati Palit.
2nd ed. 2013.
Totowa, NJ : Humana Press, 2013
X, 238 p. 51 illus., 33 illus. in color. digital.
englisch
9781627032360
10.1007/978-1-62703-236-0
Methods in molecular biology, methods and protocols ; 962
Full Text
Table of Contents:
  • Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues
  • Detecting p53 Isoforms at Protein Level
  • Autophagy, Senescence and Apoptosis
  • p53 and Cell Cycle Effects after DNA Damage
  • p53 Ubiquitination and Proteasomal Degradation.-Identification of p53 in Mitochondria.-Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis.-Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization
  • Determine the Effect of p53 on Chemosensitivity.-Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells
  • Mutant p53 in Cell Adhesion and Motility
  • Use of the DNA Fiber Spreading Technique to Detect the  Effects of Mutant p53 on DNA Replication
  • Generation of p53-deficient Induced Pluripotent Stem Cells (iPS) from Mouse Embryo Fibroblasts
  • p53 Actions on MicroRNA Expression and Maturation Pathway
  • Isolation and Characterization of Murine Multipotent Lung Stem Cells
  • Generation of p53 Knock-down Cell Lines.-ChIP for Identification of p53 Responsive DNA Promoters
  • ChIP-on-chip to Identify Mutant p53 Targets
  • ChIP Sequencing to Identify p53 Targets
  • The p53-Mdm2 Loop: A Critical Juncture of Stress Response. .