JAK-STAT Signalling [E-Book] : Methods and Protocols / edited by Sandra E. Nicholson, Nicos A. Nicola.
Nicholson, Sandra E.
Nicola, Nicos A.
Totowa, NJ : Humana Press, 2013
XI, 318 p. 52 illus., 9 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 967
Full Text
Table of Contents:
  • Analysis of Janus Tyrosine Kinase Phosphorylation and Activation
  • Co-Immunoprecipitation Protocol to Investigate Cytokine Receptor Associated Proteins e.g. Janus Kinases or Other Associated Signaling Proteins
  • In Vitro JAK Kinase Activity and Inhibition Assays
  • Quantitative Analysis of JAK Binding using Isothermal Titraion Calorimetry and Surface Plasmon Resonance
  • Determination of Protein Turnover Rates in the JAK/STAT Pathway using a Radioactive Pulse-Chase Approach
  • Designing RNAi Screens to Identify JAK/STAT Pathway Components
  • The Use of JAK-Specific Inhibitors as Chemical Biology Tools
  • Methods for Detecting Mutations in the Human JAK2 Gene
  • Detection of Activated STAT Proteins
  • Detection of Activated STAT Species Using Electrophoretic Mobility Shift Assay (EMSA) and Potential Pitfalls Arising from the Use of Detergents
  • Flow Cytometric Analysis of STAT Phosphorylation
  • Acetylation of Endogenous STAT Proteins
  • Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistochemical Staining
  • Nuclear Trafficking of STAT Proteins Visualized by Live Cell Imaging
  • Characterization of STAT Self-Association by Analytical Ultracentrifugation
  • Constitutively Active STAT5 Constructs
  • Analysis of Suppressor of Cytokine Signalling (SOCS) Gene Expression by Real-Time Quantitative PCR
  • Detection of Endogenous SOCS1 and SOCS3 Proteins by Immunoprecipitation and Western Blot Analysis
  • In vitro Ubiquitination of Cytokine Signaling Components
  • Production and Crystallization of Recombinant JAK Proteins
  • Bacterial Expression, Purification and Crystallization of Tyrosine Phosphorylated STAT Proteins.