Immune Homeostasis [E-Book] : Methods and Protocols / edited by Andrew L. Snow, Michael J. Lenardo.
Lenardo, Michael J.
Snow, Andrew L.
Totowa, NJ : Humana Press, 2013
XI, 215 p. 32 illus., 11 illus. in color. digital.
englisch
9781627032902
10.1007/978-1-62703-290-2
Methods in molecular biology, methods and protocols ; 979
Full Text
Table of Contents:
  • Quantitating Lymphocyte Programmed Cell Death (PCD) In Vitro Using Simple Kill Assays
  • Fluoresence-Activated Cell Sorting (FACS)-Based Quantitation of T Cell Receptor Restimulation-Induced Cell Death (RICD) in Activated, Primary Human T Cells
  • Evaluation of IL-2-Withdrawal Induced Apoptosis in Human T Lymphocytes
  • Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multi-Parameter Fluorescence Activated Cell Sorting (FACS)-Based Technique
  • Visualization of Fas-Mediated Death-Inducing Signaling Complex (DISC) Formation by Immunoprecipitation
  • Analyses of Programmed Cell Death in Dendritic Cells
  • Detection of Necrosis by Release of Lactate Dehydrogenase (LDH) Activity
  • Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I & II Tetramers
  • Homeostatic Proliferation of Mature T Cells
  • Quantitating Lymphocyte Homeostasis In Vivo in Humans Using Stable Isotope Tracers
  • Real-Time Quantitative (RQ-)PCR Approach to Quantify the Contribution of Proliferation to B Lymphocyte Homeostasis
  • Molecular Measurement of T Cell Receptor Excision Circles
  • Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis
  • Designs for Massively Parallel Sequencing Approaches to Identify Causal Mutations in Human Immune Disorders
  • Flow Cytometric Measurement of SLAM-Associated Protein (SAP) and X-Linked Inhibitor of Apoptosis (XIAP)
  • In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells.