Target Identification and Validation in Drug Discovery [E-Book] : Methods and Protocols / edited by Jurgen Moll, Riccardo Colombo.
Colombo, Riccardo.
Moll, Jurgen.
Totowa, NJ : Humana Press, 2013
XV, 371 p. 62 illus., 19 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 986
Full Text
Table of Contents:
  • The Path to Oncology Drug Target Validation – an Industry Perspective
  • Identification of Aptamers as Specific Binders and Modulators of Cell-Surface Receptor Activity
  • The Design and Structure-functional Properties  of DNA-based Immunomodulatory Sequences
  • siRNA Design Principles and Off-target Effects
  • Western Blot Evaluation of siRNA Delivery by pH Responsive Peptides
  • High Throughput RNAi Screening for the Identification of Novel Targets
  • Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development
  • CellProfiler and KNIME: Open Source Tools for High Content Screening
  • PARP Inhibition as a Prototype for Synthetic Lethal Screens
  • Structure-Based Target Druggability Assessment
  • Validating Pharmacological Disruption of Protein-protein Interactions by Acceptor Photobleaching FRET Imaging
  • Systematic Analysis of Complex Signal Transduction Pathways using Protein Fragment Complementation Assays
  • Reverse-Phase Protein Microarrays and Their Utility in Drug Development
  • A Cell Culture System that Mimics Chronic Lymphocytic Leukemia Cells Microenvironment for Drug Screening and Characterization
  • 2-Dimensional vs. 3-Dimensional in vitro Tumor Migration and Invasion Assays
  • Tumor Spheroid-based Migration Assays for Evaluation of Therapeutic Agents
  • The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs).-Genetically Engineered Animal Models for in vivo Target Identification and Validation in Oncology
  • Target Validation in Mice by Constitutive and Conditional RNAi
  • In vivo Target Validation by Inducible RNAi in Human Xenograft Mouse Models
  • Brightfield In Situ Hybridization Methods to Discover Gene Amplifications and Rearrangements in Clinical Samples
  • Combined microRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers.