Glycosylation Engineering of Biopharmaceuticals [E-Book] : Methods and Protocols / edited by Alain Beck
Beck, Alain
Totowa, NJ : Humana Press, 2013
XV, 355 p. 111 illus., 45 illus. in color. digital.
Methods in molecular biology, methods and protocols ; 988
Full Text
Table of Contents:
  • Engineering of Therapeutic and Diagnostic O-Glycans on Recombinant Mucin-Type Immunoglobulin Fusion Proteins Expressed in CHO Cells
  • Engineering a Human-Like Glycosylation to Produce Therapeutic Glycoproteins Based on 6-Linked Sialylation in CHO Cells
  • Glycoengineered Pichia-Based Expression of Monoclonal Antibodies
  • N-Glycosylation Humanization for Production of Therapeutic Recombinant Glycoproteins in Saccharomyces cerevisiae
  • Engineering the Baculovirus Genome to Produce Galactosylated Antibodies in Lepidopteran Cells
  • Nano LC Chips MS/MS for the Characterization of N-Glycopeptides Generated from Trypsin Digestion of a Monoclonal Antibody
  • Cetuximab Fab and Fc N-Glycan Fast Characterization Using IdeS Digestion and Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry
  • Therapeutic Antibody Glycosylation Analysis: A Contract Research Organization Perspective in the Frame of Batch Release or Comparability Support
  • Mass Spectrometric Analysis of O-Linked Oligosaccharides from Various Recombinant Expression Systems
  • Assessing Fc Glycan Heterogeneity of Therapeutic Recombinant Monoclonal Antibodies Using NP-HPLC
  • Application of Capillary Electrophoresis in Glycoprotein Analysis
  • Characterization of Glycoprotein Biopharmaceutical Products by Caliper LC90 CE-SDS Gel Technology
  • Hydrophobic Interaction Chromatography to Analyze Glycoproteins
  • Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7
  • Analysis of Monoclonal Antibodies by Sedimentation Velocity Analytical Ultracentrifugation
  • Noncovalent Mass Spectrometry for the Characterization of Antibody/Antigen Complexes
  • Conformational Analysis of Recombinant Monoclonal Antibodies with Hydrogen/Deuterium Exchange Mass Spectrometry
  • Epitope Mapping of Antibodies by Mass Spectroscopy: A Case Study
  • Evaluation of Antibody-Dependent Cell Cytotoxicity Using Lactate Deshydrogenase (LDH) Measurement
  • Evaluation of Complement Dependent Cytotoxicity Using ATP Measurement and C1q / C4b Binding
  • Capture of the Human IgG1 Antibodies by Protein A for the Kinetic Study of h-IgG / FcgR Interaction Using SPR-Based Biosensor Technology
  • Mass Spectrometry Protocol for the Absolute Quantification of a Monoclonal Antibody in Serum with Immunopurification.