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Cell Reprogramming [E-Book] : Methods and Protocols / edited by Paul J. Verma, Huseyin Sumer.

This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells, and features chapters that deal with reprogrammin...

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Sumer, Huseyin. editor
Verma, Paul J. editor
1st ed. 2015.
New York, NY : Humana Press, 2015
XI, 271 p. 27 illus., 20 illus. in color. online resource.
englisch
9781493928484
10.1007/978-1-4939-2848-4
Methods in molecular biology ; 1330
Cell biology.
Life sciences.



This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells, and features chapters that deal with reprogramming-related issues such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of reprogramming intermediates;  alternative methods for nuclear transfer; the production of germ-line chimeras from embryonic stem cells and induced pluripotent stem cells; and neonatal care and management of somatic cell nuclear transfer derived offspring.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell Reprogramming: Methods and Protocols is the perfect guide for molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.