Human Embryonic Stem Cell Protocols [E-Book] / edited by Kursad Turksen.
Turksen, Kursad. (editor)
3rd ed. 2016.
New York, NY : Humana Press, 2016
XIV, 399 p. 68 illus., 40 illus. in color. online resource.
englisch
9781493926688
10.1007/978-1-4939-2668-8
Methods in molecular biology ; 1307
Full Text
Table of Contents:
  • Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Blastocysts
  • Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC ScorecardTM Panel
  • Growth of Human Pluripotent Stem Cells Using Functional Human Extracellular Matrix
  • Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate
  • Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonic Stem Cells
  • Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR
  • Study of Gap Junctions in Human Embryonic Stem Cells
  • Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions
  • Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells
  • Genetic Manipulation of Human Embryonic Stem Cells
  • Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System
  • Use of Multicolor Flow Cytometry for Isolation of Specific Cell Populations Deriving from Differentiated Human Embryonic Stem Cells
  • Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation
  • Derivation of Endothelial Cells and Pericytes from Human Pluripotent Stem Cells
  • Differentiation of Human Embryonic Stem Cells on Periodontal Ligament Fibroblasts
  • Derivation of Epithelial Cells from Human Embryonic Stem Cells as an In Vitro Model of Vocal Mucosa
  • Human Embryonic and Hepatic Stem Cell Differentiation Visualized in Two and Three Dimensions Based on Serial Sections
  • Derivation of Chondrogenic Cells from Human Embryonic Stem Cells for Cartilage Tissue Engineering
  • Microgrooved Surface Modulates Neuron Differentiation in Human Embryonic Stem Cells
  • Directed Differentiation of Human Embryonic Stem Cells into Neural Progenitors
  • Direct Conversion of Pluripotent Human Embryonic Stem Cells Under Defined Culture Conditions into Human Neuronal or Cardiomyocyte Cell Therapy Derivatives
  • Generation of Epithelial Cell Populations from Human Pluripotent Stem Cells Using a Small Molecule Inhibitor of Src Family Kinases
  • Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells
  • Accelerated Three-Dimensional Neuroepithelium Formation from Human Embryonic Stem Cells and Its Use for Quantitative Differentiation to Human Retinal Pigment Epithelium
  • Efficient Production of Photoreceptor Precursor Cells from Human Embryonic Stem Cells
  • Generation of Megakaryocytes and Platelets from Human Pluripotent Stem Cells
  • A Simple Protocol for the Generation of Cardiomyocytes from Human Pluripotent Stem Cells.