Breast Cancer [E-Book] : Methods and Protocols / edited by Jian Cao.
Cao, Jian, (editor)
1st ed. 2016.
New York, NY : Humana Press, 2016
XII, 332 p. 71 illus., 13 illus. in color. online resource.
Methods in molecular biology ; 1406
Full Text
Table of Contents:
  • Basic Histopathological Methods and Breast Lesion Types for Research.- Clinical Applications for Immunohistochemistry of Breast Lesions.- Immunohistochemistry for Triple-Negative Breast Cancer.- In Situ Hybridization of Breast Cancer Markers.-      Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin Embedded (FFPE) Tissue Specimens by Fluorescence In Situ Hybridization (FISH).- Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR).- Detection of MiRNA in Cultured Cells or Xenograft Tissues of Breast Cancer.- Pyrosequencin g Analysis for Breast Cancer DNA Methylome.- Vita-Assay ™ Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs).- Breast Cancer Stem Cell Isolation.- Cellular Apoptosis Assay of Breast Cancer
  • Assessment of Matrix Metalloproteinases by Gel Zymography.- Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay.- Determination of Breast Cancer Cell Migratory Ability.- A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration.- Three-Dimensional Assay for Studying Breast Cancer Cell Invasion.- A Combined Phagocytosis and Fluorescent Substrate Degradation Assay to Simultaneously Assess Cell Migration and Substrate Degradation.- Analysis of Invadopodia Formation in Breast Cancer Cells.- Patient-Derived Tumor Xenograft Models of Breast Cancer.- Monitoring Phosphatidic Acid Signaling in Breast Cancer Cells Using Genetically Encoded Biosensors
  • 3D In Vitro Model for Breast Cancer Research Using Magnetic Levitation and Bioprinting Method.- Methods for Analyzing Tumor Angiogenesis in the Chick Chorioallantoic Membrane Model.- Pharmacokinetics and Pharmacodynamics in Breast Cancer Animal Models.- Intracellular Delivery of Fluorescently Labeled Polysaccharide Nanoparticles to Cultured Breast Cancer Cells.- Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression.