Plant Proteostasis [E-Book] : Methods and Protocols / edited by L. Maria Lois, Rune Matthiesen.
Lois, L. Maria, (editor)
Matthiesen, Rune, (editor)
New York, NY : Humana Press, 2016
XI, 307 p. 73 illus., 40 illus. in color. online resource.
Methods in molecular biology ; 1450
Full Text
Table of Contents:
  • Approaches to Determine Protein Ubiquitination Residue Types
  • Immunoprecipitation of Cullin Ring Ligases (CRLs) in Arabidopsis Thaliana Seedlings
  • Radioligand Binding Assays for Determining Dissociation Constants of Phytohormone Receptors
  • Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes
  • Fluorescent Reporters for Ubiquitin-Dependent Proteolysis in Plants
  • Generation of Artificial N-end Rule Substrate Proteins In Vivo and In Vitro
  • Peptide Arrays for Binding Studies of E3 Ubiquitin Ligases
  • SUMO Chain Formation by Plants Enzymes
  • Kinetic Analysis of Plant SUMO Conjugation Machinery
  • Expression, Purification, and Enzymatic Analysis Of Plant SUMO Proteases
  • Quantitative Analysis of Subcellular Distribution of the SUMO Conjugation System by Confocal Microscopy Imaging
  • Biochemical Analysis of Autophagy in Algae and Plants by Monitoring the Electrophoretic Mobility of ATG8
  • Detection of Autophagy in Plants by Fluorescence Microscopy
  • Protocols for Studying Protein Stability in a Arabidopsis Protoplast Transient Expression System
  • Detection and Quantification of Protein Aggregates in Plants
  • Determination of Protein Carbonylation and Proteasome Activity in Seeds
  • Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)-based Protein Profiling in Plants.-Use of a Phosphatidylinositol phosphate Affinity Chromatography (PIP - chromatography) for the Isolation of Proteins Involved in Protein Quality Control and Proteostasis Mechanisms in Plants
  • In vivo Radiolabeling of Arabidopsis Leaves and Separation of Thylakoid Membrane Complexes by Blue Native PAGE
  • Normalized Quantitative Western Blotting Based on Standardized Fluorescent Labeling
  • Sequence Search and Comparative Genomic Analysis of SUMO-Activating Enzymes using CoGe
  • Studying Evolutionary Dynamics of Gene Families Encoding SUMO-Activating Enzymes with SeaView and ProtTest
  • Bioinformatics Tools for Exploring the SUMO Gene Network. .