The Tumor Microenvironment [E-Book] : Methods and Protocols / edited by Josie Ursini-Siegel, Nicole Beauchemin.
Beauchemin, Nicole, (editor)
Ursini-Siegel, Josie, (editor)
New York, NY : Humana Press, 2016
XII, 356 p. 38 illus., 19 illus. in color. online resource.
englisch
9781493938018
10.1007/978-1-4939-3801-8
Methods in molecular biology ; 1458
Full Text
Table of Contents:
  • Methods of Immunohistochemistry and Immunofluorescence: Converting Invisible to Visible
  • Laser Capture Microdissection as a Tool to Study Tumor Stroma
  • Quantitative Analysis of Human Cancer Cell Extravasation Using Intravital Imaging
  • Studies on the Tumor Vasculature and Coagulant Microenvironment
  • A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment
  • Measuring Vascular Permeability In Vivo
  • Hydroxylation-Dependent Interaction of Substrates to the Von Hippel-Lindau Tumor Suppressor Protein (VHL)
  • Analyzing the Tumor Microenvironment by Flow Cytometry
  • Detecting Secreted Analytes from Immune Cells: An Overview of Technologies
  • Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-Parameter Flow Cytometry-Based Sorting
  • Viral Engineering of Chimeric Antigen Receptor Expression on Murine and Human T Lymphocytes
  • Methods to Evaluate the Anti-Tumor Activity of Immune Checkpoint Inhibitors in Preclinical Studies
  • Isolation and Characterization of Low Vs. High-Density Neutrophils in Cancer
  • Analysis of Extracellular Vesicles in the Tumor Microenvironment
  • Visualizing the Tumor Microenvironment of Liver Metastasis by Spinning Disc Confocal Microscopy
  • Intravital Microscopy for Imaging the Tumor Microenvironment in Live Mice
  • Development of a Patient-Derived Xenograft Model Using Brain Tumor Stem Cell Systems to Study Cancer
  • Modeling Breast Tumor Development with a Humanized Mouse Model
  • CRISPR/Cas9 Genome Editing as a Strategy to Study the Tumor Microenvironment in Transgenic Mice
  • Metabolomics Analyses of Cancer Cells in Controlled Microenvironments
  • Analysis of the Tumor Microenvironment Transcriptome Via NanoString mRNA and miRNA Expression Profiling
  • RNA-Seq as a Tool to Study the Tumor Microenvironment
  • Sample Preparation for Mass Spectrometry Analysis of Protein-Protein Interactions in Cancer Cell Lines and Tissues.