Wnt Signaling [E-Book] : Methods and Protocols / edited by Quinn Barrett, Lawrence Lum.
Barrett, Quinn, (editor)
Lum, Lawrence, (editor)
New York, NY : Humana Press, 2016
XI, 198 p. 45 illus., 32 illus. in color. online resource.
englisch
9781493963935
10.1007/978-1-4939-6393-5
Methods in molecular biology ; 1481
Full Text
Table of Contents:
  • Visualizing Wnt Palmiteoylation in Single Cells
  • Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction
  • Biochemical Methods to Analyze Wnt Protein Secretion
  • Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum
  • Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway
  • A Simple Method to Assess Abundance of the b-Catenin Signaling Pool in Cells
  • Wnt-Dependent Control of Cell Polarity in Cultured Cells
  • The Use of Chick Embryos to Study Wnt Signaling/Gradients
  • Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors
  • Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo
  • Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts
  • Delivery of the Porcupine Inhibitor WNT974 in Mice
  • Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis
  • Monitoring Wnt/b-Catenin Signaling in Skin
  • The Generation of Organoids for Studying Wnt Signaling
  • Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells
  • Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions.