Synthetic DNA [E-Book] : Methods and Protocols / edited by Randall A. Hughes.
Hughes, Randall A., (editor)
New York, NY : Humana Press, 2017
XIII, 248 p. 74 illus., 60 illus. in color. online resource.
englisch
9781493963430
10.1007/978-1-4939-6343-0
Methods in molecular biology ; 1472
Full Text
Table of Contents:
  • A Guide to Using STITCHER for Overlapping Assembly PCR Applications
  • Synthetic Gene Design Using Codon Optimization On-Line (COOL)
  • Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineering
  • Simple Cloning by Prolonged Overlap Extension PCR with Application to the Preparation of Large-Size Random Gene Mutagenesis Library in Escherichia coli
  • SpeedyGenes: Exploiting an Improved Gene Synthesis Method for the Efficient Production of Error-corrected, Synthetic Protein Libraries for Directed Evolution
  • BASIC A Simple and Accurate Modular DNA Assembly Method
  • Enzymatic Synthesis of Single Stranded Clonal Pure Oligonucleotides
  • Rapid Assembly of DNA via Ligase Cycling Reaction (LCR)
  • PaperClip: A Simple Method for Flexible Multi-part DNA Assembly
  • The Polymerase Step Reaction (PSR) Method for Gene and Library Synthesis
  • Clonetegration Using OSIP Plasmids One Step DNA Assembly and Site Specific Genomic Integration in Bacteria
  • Generation of DNA Constructs Using the Golden GATEway Cloning Method
  • Gene Deletion by Synthesis in Yeast
  • Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR)
  • Simultaneous Removal of Multiple DNA Segments by Polymerase Chain Reactions
  • Rapid Construction of Recombinant Plasmids by QuickStep-Cloning
  • Immobilized MutS-mediated Error Removal of Microchip-synthesized DNA
  • Selection of Error-less Synthetic Genes in Yeast.