Optical Tweezers [E-Book] : Methods and Protocols / edited by Arne Gennerich.
Gennerich, Arne, (editor)
New York, NY : Humana Press, 2017
XII, 555 p. 154 illus., 133 illus. in color. online resource.
englisch
9781493964215
10.1007/978-1-4939-6421-5
Methods in molecular biology ; 1486
Full Text
Table of Contents:
  • Introduction to Optical Tweezers
  • Exact Theory of Optical Tweezers and its Application to Absolute Calibration
  • Beyond the Hookean Spring Model: Direct Measurement of Optical Forces Through Light Momentum Changes
  • A Surfaced-Coupled Optical Trap with 1-bp Precision via Active Stabilization
  • Implementation and Tuning of an Optical Tweezers Force-Clamp Feedback System
  • Custom-Made Microspheres for Optical Tweezers
  • Optical Torque Wrench Design and Calibration
  • High-Resolution Fleezers: Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection
  • Versatile Quadruple-Trap Optical Tweezers for Dual DNA Experiments
  • Probing DNA-DNA Interactions with a Combination of Quadruple-Trap Optical Tweezers and Microfluidics
  • Probing Single Helicase Dynamics on Long Nucleic Acids Through Force-Fluorescence Measurement
  • Mechanically Watching the ClpXP Proteasome Machinery
  • Deciphering the Molecular Mechanism of the Bacteriophage 29 DNA Packaging Motor
  • Single-Molecule Protein Folding Experiments using High-Precision Optical Tweezers
  • Observing Single RNA Polymerase Molecules Down to Base-Pair Resolution
  • Optical Tweezers-Based Measurements of Forces Generated by Dynamic Microtubule Ends
  • Simultaneous Manipulation and Super-Resolution Fluorescence Imaging of Individual Kinetochores Coupled to Microtubule Tips
  • Measurement of Force-Dependent Release Rates of Cytoskeletal Motors
  • Measuring The Kinetic and Mechanical Properties of Non-Processive Myosins using Optical Tweezers
  • Quantifying Force and Viscoelasticity Inside Living Cells Using an Active-Passive Calibrated Optical Trap
  • Measuring Molecular Forces using Calibrated Optical Traps in Living Cells. .