T-Cell Differentiation [E-Book] : Methods and Protocols / edited by Enrico Lugli.
Lugli, Enrico, (editor)
New York, NY : Humana Press, 2017
XI, 271 p. 55 illus., 44 illus. in color. online resource.
Methods in molecular biology ; 1514
Full Text
Table of Contents:
  • The Vast Universe of T Cell Diversity: Subsets of Memory Cells and their Differentiation
  • Basic Aspects of T Helper Cell Differentiation
  • FACS Analysis of Memory T lymphocytes
  • Intravital Microscopy Analysis of Hepatic T cell dynamics
  • Analysis of T Cell Activation by Confocal Microscopy
  • Phenotypic and Functional Analysis of Antigen-specific T Cell Exhaustion
  • pMHC Multiplexing Strategy to Detect High Numbers of T Cell Responses in Parallel
  • Differentiation of Diverse Progenies of Memory T cells from Naïve CD8+ T Cell Precursors
  • Gammaretroviral Production and T Cell Transduction To Genetically Retarget Primary T Cells Against Cancer
  • Measuring Telomerase Activity in Senescent Human T Cells upon Genetic Modification
  • Strategies for T Helper Cell Subset Differentiation from Naïve Precursors
  • Phenotypic and Functional Analysis of the Suppressive Function of Human Regulatory T Cells
  • Approaches to Detect microRNA Expression in T Cell Subsets and T Cell Differentiation
  • Next-generation Sequencing Analysis of Long Non-Coding RNAs in CD4+ T Cell Differentiation
  • Highly Multiplexed, Single Cell Transcriptomic Analysis of T-cells by Microfluidic PCR
  • Single-cell RNA Sequencing of Human T Cells
  • Extensive Phenotypic Analysis, Transcription Factor Profiling, and Effector Cytokine Production of Human MAIT Cells by Flow Cytometry
  • Developmental and Functional Assays to Study Murine and Human gd T Cells. .