Histones [E-Book] : Methods and Protocols / edited by Benoit Guillemette, Luc R. Gaudreau.
Gaudreau, Luc R., (editor)
Guillemette, Benoit, (editor)
New York, NY : Humana Press, 2017
X, 279 p. 36 illus., 17 illus. in color. online resource.
Methods in molecular biology ; 1528
Full Text
Table of Contents:
  • In Vitro Assembly of Nucleosomes For Binding/Remodeling Assays
  • An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro
  • Purification of Yeast Native Reagents for the Analysis of Chromatin Function-I: Nucleosomes for Reconstitution and Manipulation of Histone Marks
  • Purification of Yeast Native Reagents for the Analysis of Chromatin Function-II: Multiprotein Complexes and Biochemical Assays
  • Histone Purification from Saccharomyces Cerevisiae
  • Analytical Ultracentrifuge Analysis of Nucleosomes Assembled from Recombinant, Acid Extracted, Hplc-Purified Histones
  • Silac-Based Quantitative Strategies for Accurate Histone Post-Translational Modifications Profiling Across Multiple Biological Samples
  • Characterization of Individual Histone Post-Translational Modifications and their Combinatorial Patterns by Mass Spectrometry-Based Proteomics Strategies
  • Production and Purification of Antibodies against Histone Modifications
  • Immunofluorescence of Histone Proteins
  • Acid-Urea Gel Electrophoresis and Western Blotting Of Histones
  • Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast
  • A Spiking Strategy for Chip-Chip Data Normalization in S. Cerevisiae
  • High-Resolution Genome-Wide Mapping Of Nucleosome Positioning And Occupancy Level Using Paired-End Sequencing Technology
  • Physarum Polycephalum for Studying the Function Of Histone Modifications In Vivo
  • A Method for Large Scale Screening of Random Sequence Libraries to Determine the Function of Unstructured Regions from Essential Proteins. .