Hydrocarbon and Lipid Microbiology Protocols [E-Book] : Genetic, Genomic and System Analyses of Communities / edited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales.
This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. Methods are described for the creation and bioinformatic assessment of metagenomic and metatranscriptomic libraries, and for metaproteomic analyses, whic...
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Personal Name(s): | McGenity, Terry J., editor |
Nogales, Balbina, editor / Timmis, Kenneth N., editor | |
Edition: |
1st edition |
Imprint: |
Berlin, Heidelberg :
Springer,
2017
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Physical Description: |
X, 288 p. 50 illus., 30 illus. in color. online resource. |
Note: |
englisch |
ISBN: |
9783662504505 |
DOI: |
10.1007/978-3-662-50450-5 |
Series Title: |
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Springer Protocols handbooks
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Classification: |
- Introduction to genetic, genomic and system analyses for communities
- Genomic analysis of pure cultures and communities
- Protocols for metagenomic library generation and analysis in petroleum hydrocarbon microbe systems
- Preparation and analysis of metatranscriptomic libraries in petroleum hydrocarbon microbe systems
- Prokaryotic metatranscriptomics
- Defining a pipeline for metaproteomic analyses
- Generating enriched metagenomes from active microorganisms with DNA stable isotope probing
- DNA and RNA-based stable isotope probing of hydrocarbon degraders
- Protein stable isotope probing (Protein-SIP)
- Protein extraction from contaminated soils and sediments
- Analysis of the regulation of the rate of hydrocarbon and nutrient flow through microbial communities
- Physiological modelling of complex bacterial communities
- Protocol for evaluating the permissiveness of bacterial communities towards conjugal plasmids by quantification and isolation of transconjugants.