Hydrocarbon and Lipid Microbiology Protocols [E-Book] : Microbial Quantitation, Community Profiling and Array Approaches / edited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales.
McGenity, Terry J., (editor)
Nogales, Balbina, (editor) / Timmis, Kenneth N., (editor)
1st edition
Berlin, Heidelberg : Springer, 2017
X, 252 p. 30 illus., 12 illus. in color. online resource.
Springer Protocols handbooks
Full Text
Table of Contents:
  • Introduction to Microbial Quantitation, Community Profiling, and Array Approaches
  • A cell extraction method for oily sediments
  • Introduction to microplate MPN-enumeration of hydrocarbon degraders
  • Primers for dsr genes and Most Probable Number Method for detection of sulfate-reducing bacteria in oil reservoirs
  • Microbial biomass and community analysis by PLFA analysis
  • Real-time PCR approaches for analysis of hydrocarbon-degrading bacterial communities
  • Clone libraries of ribosomal RNA gene sequences for characterization of microbial communities
  • Denaturing gradient gel electrophoresis (DGGE) for microbial community analysis
  • Microbial community profiling: SSCP and T-RFLP techniques
  • Microbial community analysis by single-amplicon high-throughput sequencing (metagenetics) preparation, data handling and ecological analysis
  • Multiplex fluorescent antibody microarrays and antibody graphs for microbial and biomarker detection in the environment
  • Studying protistan communities in hydrocarbon-contaminated environments.