SH2 Domains [E-Book] : Methods and Protocols /

SH2 Domains [E-Book] : Methods and Protocols / edited by Kazuya Machida, Bernard A. Liu.

This volume covers an array of techniques available for studying SH2 domains and phosphotyrosine signaling. The book is divided into six parts: Part I outlines the history of SH2, technology development, and cell signaling; Part II focuses on computational approaches and tools used for identificatio...

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Liu, Bernard A., editor
Machida, Kazuya, editor
New York, NY : Humana Press, 2017
XIV, 558 p. 118 illus., 79 illus. in color. online resource.
englisch
9781493967629
10.1007/978-1-4939-6762-9
Methods in molecular biology ; 1555
domain structure
protein analysis
Life sciences.
Proteins.
LLH - Protein chemistry - methods



  • Introduction: History of SH2 Domains and Their Applications
  • What Have We Learned from SH2 Domains?
  • Hidden Markov Models for Protein Domain Homology Identification and Analysis
  • Classification and Lineage Tracing of SH2 Domains Throughout Eukaryotes
  • SH2 Ligand Prediction-Guidance for In-Silico Screening
  • An Efficient Semi-Supervised Learning Approach to Predict SH2 Domain Mediated Interactions
  • Proteomic Clustering Analysis of SH2 Domain Datasets
  • Expression and Production of SH2 Domain Proteins
  • Expression and Purification of Soluble STAT5b/STAT3 Proteins for SH2 Domain Binding Assay
  • Purification of SOCS (Suppressors of Cytokine Signalling) SH2 Domains for Structural and Functional Studies
  • Expression and Purification of SH2 Domains Using Baculovirus Expression System
  • Functionally Altered SH2 Domains for Biochemical Studies: Loss of Function Mutant and Domain Concatenation
  • Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domain
  • Structural Characterization of Monomeric/Dimeric State of p59fyn SH2 Domain
  • NMR Chemical Shift Mapping of SH2 Peptide Interactions
  • Calorimetric Measurement of SH2 Domain Ligand Affinities
  • Binding Assays Using Recombinant SH2 Domains: Far-Western, Pull Down, and Fluorescence Polarization
  • In-Solution SH2 Domain Binding Assay Based on Proximity Ligation
  • Alpha-Based Multiplexed Assay for Identifying SH2 Domain Antagonists
  • Characterizing SH2 Domain Specificity and Network Interactions Using SPOT Peptide Arrays
  • High Throughput Quantification of SH2 Domain-Phosphopeptide Interactions with Cellulose Peptide Conjugate Microarrays
  • SH2 Domains as Affinity Reagents for Phosphotyrosine Protein Enrichment and Proteomic Analysis
  • Identification of Tyrosine Phosphorylated Proteins by SH2 Domain Affinity Purification and Mass Spectrometry
  • Analysis of the Global Changes in SH2 Binding Properties Using Mass Spectrometry Supported by Quantitative Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technique
  • Using Reciprocal Protein-Peptide Array Screening to Unravel Protein Interaction Networks
  • Rosette Assay: Highly Customizable Dot-Blot for SH2 Domain Screening
  • Microwestern Arrays for Systems-Level Analysis of SH2 Domain-Containing Proteins
  • SH2 Binding Site Protection Assay: A Method for Identification of SH2 Domain Interaction Partners by Exploiting SH2 Mediated Phosphosite Protection
  • Real Time Single Molecule Visualization of SH2 Domain Membrane Recruitment in Growth Factor Stimulated Cells
  • SH2 Domain-Based FRET Biosensor for Measuring BCR-ABL Activity in Living CML Cells
  • SH2 Domain Histochemistry.