Thermogenic Fat [E-Book] : Methods and Protocols / edited by Jun Wu.
Wu, Jun, (editor)
New York, NY : Humana Press, 2017
XI, 214 p. 42 illus., 30 illus. in color. online resource.
Methods in molecular biology ; 1566
Full Text
Table of Contents:
  • Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue
  • Isolation of Mouse Stromal Vascular Cells for Monolayer Culture
  • Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells
  • Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes
  • Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells
  • Isolation of Immune Cells from Adipose Tissue
  • Differentiation and Metabolic Interrogation of Human Adipocytes
  • Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines
  • Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue
  • Genetic Mouse Models: The Powerful Tools to Study Fat Tissues
  • Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function
  • Bioenergetic Analyses in Adipose Tissue
  • Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry
  • Isolation and Patch-Clamp of Primary Adipocytes
  • In Vitro Approaches to Model and Study Communications Between Adipose Tissue and the Liver
  • Identification and Quantification of Human Brown Adipose Tissue
  • Designing 3D Adipospheres for Quantitative Metabolic Study
  • Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems
  • Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides.