Synthetic Antibodies [E-Book] : Methods and Protocols / edited by Thomas Tiller.
Tiller, Thomas, (editor)
New York, NY : Humana Press, 2017
XIV, 420 p. 102 illus., 74 illus. in color. online resource.
englisch
9781493968572
10.1007/978-1-4939-6857-2
Methods in molecular biology ; 1575
Full Text
Table of Contents:
  • Antibody Mimetics, Peptides, and Peptidomimetics
  • Construction of a scFv Library with Synthetic, Non-Combinatorial CDR Diversity
  • Enzymatic Assembly for scFv Library Construction
  • Directed Evolution of Protein Thermal Stability Using Yeast Surface Display
  • Whole Cell Panning with Phage Display
  • Generating Conformation and Complex-Specific Synthetic Antibodies
  • High-Throughput IgG Conversion of Phage Displayed Fab Antibody Fragments by AmplYFast
  • Utilization of Selenocysteine for Site-Specific Antibody Conjugation
  • Solubility Characterization and Imaging of Intrabodies Using GFP-Fusions
  • Antibody Validation by Immunoprecipitation Followed by Mass Spectrometry Analysis
  • Novel HPLC-Based Screening Method to Assess Developability of Antibody-Like Molecules
  • Glycosylation Profiling of / T Cell Receptor Constant Domains Expressed in Mammalian Cells
  • A Proximity-Based Assay for Identification of Ligand and Membrane Protein Interaction in Living Cells
  • A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteins
  • Data-Driven Antibody Engineering Using Genedata Biologics
  • Selection of Aptamers Against Whole Living Cells: From Cell-SELEX to Identification of Biomarkers
  • Rapid Selection of RNA Aptamers that Activate Fluorescence of Small Molecules
  • An Enzyme-Linked Aptamer Sorbent Assay to Evaluate Aptamer Binding
  • Incorporating Aptamers in the Multiple Analyte Profiling Assays (xMAP): Detection of C-Reactive Protein
  • Transferring the Selectivity of a Natural Antibody into a Molecularly Imprinted Polymer
  • Preparation of Molecularly Imprinted Microspheres by Precipitation Polymerization
  • Generation of Janus Molecularly Imprinted Polymer Particles
  • Surface Engineering of Nanoparticles to Create Synthetic Antibodies
  • H5N1 Virus Plastic Antibody Based on Molecularly Imprinted Polymers
  • Replacement of Antibodies in Pseudo-ELISAs: Molecularly Imprinted Nanoparticles for Vancomycin Detection
  • Cell and Tissue Imaging with Molecularly Imprinted Polymers.