Erythropoiesis [E-Book] : Methods and Protocols / edited by Joyce A. Lloyd.
This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to analyze circulating red blood cell parameters,...
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Full text |
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Personal Name(s): | Lloyd, Joyce A., editor |
Imprint: |
New York, NY :
Humana Press,
2018
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Physical Description: |
XI, 298 p. 62 illus., 55 illus. in color. online resource. |
Note: |
englisch |
ISBN: |
9781493974283 |
DOI: |
10.1007/978-1-4939-7428-3 |
Series Title: |
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Methods in molecular biology ;
1698 |
Subject (ZB): | |
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- An Introduction to Erythropoiesis Approaches
- Using the Zebrafish as an Approach to Examine the Mechanisms of Vertebrate Erythropoiesis
- Mouse Models of Erythropoiesis and Associated Diseases
- Dissecting Regulatory Mechanisms Using Mouse Fetal Liver-Derived Erythroid Cells
- Stress Erythropoiesis Model Systems
- Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models
- Functional Analysis of Erythroid Progenitors by Colony-Forming Assays
- Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands
- Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells
- Analysis of Erythropoiesis Using Imaging Flow Cytometry
- Flow Cytometric Analysis of Erythroblast Enucleation
- High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure
- Chromatin Immunoprecipitation (ChIP) with Erythroid Samples
- Chromosome Conformation Capture (3C and Higher) with Erythroid Samples
- Genome Editing of Erythroid Cell Culture Model Systems
- In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Cord Blood
- Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines
- Good Manufacturing Practice (GMP) Translation of Advanced Cellular Therapeutics: Lessons for the Manufacture of Erythrocytes as Medicinal Products.