Schizosaccharomyces pombe [E-Book] : Methods and Protocols / edited by Teresa L. Singleton.
Singleton, Teresa L., (editor)
New York, NY : Humana Press, 2018
XII, 244 p. 48 illus., 34 illus. in color. online resource.
Methods in molecular biology ; 1721
Full Text
Table of Contents:
  • Preparation of Solutions and Reagents
  • Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale using DNA Combing
  • Chromatin Immunoprecipitation - Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe
  • Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs
  • Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast
  • Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast
  • Analysis of Reverse Transcribed mRNA using PCR and Polyacrylamide Gel Electrophoresis
  • The No-nonsens SDS-PAGE
  • Crystallization of Recombinant -actinin and Related Proteins
  • Estimation of GFP-nucleoporin Amount Based on Fluorescence Microscopy
  • Antibody Pull-down Experiments in Fission Yeast
  • Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation
  • Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo
  • In Situ Chromatin Binding Assay Using Epifluorescent Microscopy in S. pombe
  • High-frequency Lithium Acetate Transformation of Schizosaccharomyces pomTetrad Dissection in Fission Yeast
  • Random Spore Analysis in Fission Yeast
  • Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains
  • Schizosaccharomyces pombe Biotechnological Applications in Winemaking
  • Schizosaccharomyces pombe Isolation Protocol..