Noncanonical Amino Acids [E-Book] : Methods and Protocols / edited by Edward A. Lemke.
Lemke, Edward A., (editor)
New York, NY : Humana Press, 2018
XI, 411 p. 76 illus., 39 illus. in color. online resource.
englisch
9781493975747
10.1007/978-1-4939-7574-7
Methods in molecular biology ; 1728
Full Text
Table of Contents:
  • Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugates
  • Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypeptide Synthesis
  • Cell-free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 deletion E. coli Strains
  • Tub-tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acids
  • Directed Evolution of Orthogonal Pyrrolysyl-tRNA Synthetases in Escherichia coli for the Genetic Encoding of Noncanonical Amino Acids
  • Genetic Code Expansion in Enteric Bacterial Pathogens
  • Self-directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and its Incorporation into Model Proteins
  • Residue-specific Incorporation of no Canonical Amino Acids for Protein Engineering
  • Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene
  • Genetic Incorporation of Unnatural Amino Acids into Proteins of Interest in Streptomyces venezuelae ATCC 15439
  • Expression and Purification of Site-specifically Lysine-acetylated and Natively-folded Proteins for Biophysical Investigations
  • Site-specific Incorporation of Sulfotyrosine Using an Expanded Genetic Code
  • Site-specific Protein Labeling With Tetrazine Amino Acids
  • Mapping of Protein Interfaces in Live Cells Using Genetically Encoded Crosslinkers
  • Generation of Stable Amber Suppression Cell Lines
  • Trapping Chromatin Interacting Proteins with Genetically Encoded, UV-Activatable Crosslinkers In Vivo
  • Genetically Encoding Unnatural Amino Acids in Neurons in vitro and in the Embryonic Mouse Brain for Optical Control of Neuronal Proteins
  • Genetic Code Expansion-and Click Chemistry-Based Site
  • Specific Protein Labelling For Intracellular DNA
  • PAINT Imaging
  • MultiBacTAG- Genetic Code Expansion using the Baculovirus Expression System in Sf21 Cells
  • Production and Chemoselective Modification of Adeno-associated Virus Site-specifically Incorporating an Unnatural Amino Acid Residue into its Capsid
  • Generation of Intramolecular FRET Probes via Noncanonical Amino Acid Mutagenesis
  • Fluorogenic Tetrazine
  • siliconrhodamine Probe for the Labeling of Non-canonical Amino Acid Tagged Proteins
  • Site-specific Protein Labeling Utilizing Lipoic Acid Ligase (LplA) and Bioorthogonal Inverse Electron Demand Diels-Alder Reaction
  • Genetic Encoding of Unnatural Amino Acids in C. elegans.