Bacterial Regulatory RNA [E-Book] : Methods and Protocols / edited by Véronique Arluison, Claudio Valverde.
Arluison, Véronique, (editor)
Valverde, Claudio, (editor)
New York, NY : Humana Press, 2018
XII, 395 p. 84 illus., 62 illus. in color. online resource.
Methods in molecular biology ; 1737
Full Text
Table of Contents:
  • Workflow for a Computational Analysis of an sRNA Candidate in Bacteria
  • Molecular Phylogenomics for the Identification and Characterization of trans-encoded Small Non-coding RNA Families
  • Bioinformatic Approach for Prediction of CsrA/RsmA-regulating small RNAs in Bacteria
  • Host-pathogen Transcriptomics by Dual RNA-seq
  • Identification of New Bacterial Small RNA Targets using MS2 Affinity Purification Coupled to RNA Sequencing
  • Assessment of External Guide Sequences (EGS) Efficiency as Inducers of RNase P-mediated Cleavage of mRNA Target Molecules
  • Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-dependent Termination of Transcription in vitro
  • A Method to Map Changes in Bacterial Surface Composition Induced by Regulatory RNAs
  • Fluorescence-based Methods for Characterizing RNA interactions in vivo
  • Mutational Analysis of sRNA-mRNA Base Pairing by Electrophoretic Mobility Shift Assay
  • An Integrated Cell-free Assay to Study Translation Regulation by Small Bacterial Non-coding RNAs
  • Quantitative Super-resolution Imaging of Small RNAs in Bacterial Cells
  • Extraction and Analysis of RNA Isolated from Pure BacteriaDerived outer Membrane Vesicles
  • Absolute Regulatory Small Noncoding RNA Concentration and Decay Rates Measurements in Escherichia coli
  • High-resolution, High-throughput Analysis of Hfq Binding Sites using UV-crosslinking and Analysis of cDNA (CRAC)
  • Producing Hfq/Sm Proteins and sRNAs for Structural and Biophysical Studies of Ribonucleoprotein Assembly
  • Single Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing
  • Techniques to Analyse sRNA-protein Cofactor Self-assembly in vitro
  • Sequence-specific Affinity Chromatography of RNA-binding Proteins from Bacterial Cells
  • Identification of Small RNA-protein Partners in Plant Symbiotic Bacteria
  • A Modular Genetic System for High-throughput Profiling and Engineering of Multi-target Small RNAs..