Progranulin [E-Book] : Methods and Protocols / edited by Andrew Bateman, Hugh P.J. Bennett, Siu Tim Cheung.
Bateman, Andrew, (editor)
Bennett, Hugh P.J., (editor) / Cheung, Siu Tim, (editor)
New York, NY : Humana Press, 2018
XIII, 363 p. 79 illus., 56 illus. in color. online resource.
englisch
9781493985593
10.1007/978-1-4939-8559-3
Methods in Molecular Biology ; 1806
Full Text
Table of Contents:
  • A Brief Overview of Progranulin in Health and Disease
  • Chromatographic Methods for the Purification of Granulin Peptides
  • Methods for Expression and Purification of Biologically Active Recombinant Progranulin
  • Large-Scale Generation of Recombinant Granulin Peptides in E. coli
  • Nuclear Magnetic Resonance Spectroscopy in Analysis of Granulin Three-Dimensional Structure and Cysteine Bridging
  • Data Mining: Applying the AD&FTD Mutation Database to Progranulin
  • Measurement of Circulating Progranulin (PGRN/GP88/GEP) by Enzyme-Linked Immunosorbent Assay and Application in Human Diseases
  • Immunohistochemical Detection of Progranulin (PGRN/GP88/GEP) in Tumor Tissues as a Cancer Prognostic Biomarker
  • Analysis of Progranulin-Mediated Akt and MAPK Activation
  • Mouse Monoclonal Antibodies Against Progranulin (PGRN/GEP) as Therapeutics in Preclinical Cancer Models
  • Methods to Analyze the Role of Progranulin (PGRN/GEP) on Cancer Stem Cell Features
  • Methods to Study the Role of Progranulin in the Tumor Microenvironment
  • Methods to Investigate the Molecular Basis of Progranulin Action on Neurons In Vivo using Caenorhabditis elegans
  • The Use of Caenorhabditis elegans to Study Progranulin in the Regulation of Programmed Cell Death and Stress Response
  • Application of Zebrafish and Knockdown Technology to Define Progranulin Neuronal Function
  • Methods to Investigate the Molecular Basis of Progranulin Actions on Brain and Behavior In Vivo Using Knockout Mice
  • Methods to Investigate the Protection Againts Neurodegenerative Disorders Provided by Progranulin Gene Transfer in the Brain
  • The Interaction between Progranulin with Sortilin and the Lysosome
  • Methods to Study the Role of Progranulin in Preimplanatation Mouse Embryo Development
  • Establishment of a Modified Collagen-Induced Arthritis Mouse Model to Investigate the Anti-Inflammatory Activity of Progranulin in Inflammatory Arthritis
  • Methods for Studying the Function of Progranulin in Atherosclerosis using Both Knockout Mice Models and In Vitro Studies
  • Methods to Investigate the Roles of Prgranulin in Angiogenesis Using In Vitro Strategies and Transgenic Mouse Models.