Planarian Regeneration [E-Book] : Methods and Protocols / edited by Jochen C. Rink.
Rink, Jochen C., (editor)
New York, NY : Humana Press, 2018
XI, 573 p. 92 illus., 56 illus. in color. online resource.
englisch
9781493978021
10.1007/978-1-4939-7802-1
Methods in Molecular Biology ; 1774
Full Text
Table of Contents:
  • Planarian Diversity and Phylogeny
  • Stem Cells, Patterning, and Regeneration in Planarians-Self-Organization at the Organismal Scale
  • The Ecology of Freshwater Planarians
  • Planarians and the History of Animal Regeneration: Paradigm Shifts and Key Concepts in Biology
  • Culturing Planarians in the Laboratory
  • Total RNA Isolation from Planarian Tissues
  • Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians
  • Planarian High Molecular Weight DNA Isolation by Spooling
  • Basic Histological Techniques for Planarians
  • Fixation, Processing, and Immunofluorescent Labeling of Whole Mount Planarians
  • Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections
  • Whole-Mount In Situ Hybridization of Planarians
  • RNA In Situ Hybridization on Planarian Paraffin Sections
  • General Principles of Planarian Embryogenesis and Its Analysis by In Situ Hybridization and Immunohistochemistry Methods
  • Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians
  • Detection of Apoptotic Cells in Planarians by Whole-Mount TUNEL
  • Systemic RNA Interference in Planarians by Feeding of dsRNA Containing Bacteria
  • RNA Interference in Planarians: Feeding and Injection of Synthetic dsRNA
  • A Subtractive FACS Method for Isolation of Planarian Stem Cells and Neural Cells
  • Clonal Analysis of Planarian Stem Cells by Subtotal Irradiation and Single-Cell Transplantation
  • Tissue Transplantations in Planarians
  • Live Imaging Planaria
  • Processing Schmidtea mediterranea for Transmission Electron Microscopy: Classical and Microwave Techniques
  • Tissue Extracts for Quantitative Mass Spectrometry of Planarian Proteins Using SILAC
  • Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method.