Pre-Clinical Models [E-Book] : Techniques and Protocols /

Pre-Clinical Models [E-Book] : Techniques and Protocols / edited by Paul C. Guest.

This volume details reviews and protocols on the development and analysis of both cellular and animal-based pre-clinical models in a number of medical areas, including metabolic disorders, longevity, cancer, heart disease and psychiatric disorders. Written in the highly successful Methods in Molecul...

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Guest, Paul C., editor
New York, NY : Humana Press, 2019
XIII, 343 pages 90 illustrations, 73 illustrations in color (online resource)
englisch
9781493989942
10.1007/978-1-4939-8994-2
Methods in Molecular Biology ; 1916
Laboratory animals.
Toxicology.



  • A Guide to Mass Spectrometry-based Quantitative Proteomics
  • Mitochondrial Involvement in Mental Disorders; Energy Metabolism, Genetic and Environmental Factors
  • Monosodium Glutamate (MSG)-induced Animal Model of Type 2 Diabetes
  • The Elevated Plus Maze Test for Measuring Anxiety-like Behaviour in Rodents
  • The Forced Swim Test for Depression-like Behaviour in Rodents
  • Object Burying Test for Assessment of Obsessive Compulsive Behaviours in Mice
  • The Nest Building Test in Mice for Assessment of General Well-being
  • Mouse Free Dyadic Social Interaction Test
  • The Open Field Test for Measuring Locomotor Activity and Anxiety-Like Behaviour
  • The Y-maze for Assessment of Spatial Working and Reference Memory in Mice
  • Maturation of a Human Oligodendrocyte Cell Line
  • Monitoring Age-related Changes in the Lactate/pyruvate Ratio using a Colorimetric Assay in a C. elegans Model of Increased Lifespan
  • Isolation and Characterisation of Different Mesenchymal Stem Cell Populations from Rat Femur
  • Characterization of an Animal Model of Autism and Social Interaction
  • Assaying Reproductive Capacity in Female Rodents
  • Experimental Model of Hindlimb Suspension-Induced Skeletal Muscle Atrophy in Rodents
  • An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes using Flow Cytometry
  • Characterization of the db/db Mouse Model of Type 2 Diabetes
  • Characterization of the Goto-Kakizaki (GK) Rat Model of Type 2 Diabetes
  • Characterization of Human Transplantable Insulinoma Cells
  • Preparation of Islets from Rat Pancreas and Assessment of Islet Function
  • Cryopreservation of Red Blood Cells
  • Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryo-preservation
  • Reprogramming of Human Melanocytes and Melanoma cells with Yamanaka Factors
  • The Use of 3T3-L1 Murine Preadipocytes as a Model of Adipogenesis
  • Generation of Human Pyruvate Carboxylase Knockout Cell Lines Using Retrovirus Expressing Short Hairpin RNA and CRISPR-Cas9 as Models to Study its Metabolic Role in Cancer Research
  • The use of Primary Hepatocytes in Assessment of Drug Safety and Toxicity
  • OVA-induced Allergic Airway Inflammation Mouse Model
  • Description of Ovariectomy Protocol in Mice
  • A Rat Eye Lens Model of Cataract Formation
  • A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue
  • Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 β-Cell Line- a Preclinical Model for Type 2 Diabetes.