Bacterial Polysaccharides [E-Book] : Methods and Protocols / edited by Inka Brockhausen.
This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthes...
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Full text |
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Personal Name(s): | Brockhausen, Inka, editor |
Imprint: |
New York, NY :
Humana Press,
2019
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Physical Description: |
XIII, 312 pages 70 illustrations, 41 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781493991549 |
DOI: |
10.1007/978-1-4939-9154-9 |
Series Title: |
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Methods in Molecular Biology ;
1954 |
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Classification: |
- 1. Customisable cloning of whole polysaccharide gene clusters by yeast homologous recombination
- 2. RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-negative Bacteria
- 3. Purification of Capsular Polysaccharide Complex from Gram Negative Bacteria
- 4. Simple protocol to purify cell wall polysaccharide from Gram+ bacteria and assess its structural integrity
- 5. Extraction and purification of wall bound polymers of Gram-positive bacteria
- 6. Purification and Analysis of Mycobacterial Phosphatidylinositol Mannosides, Lipomannan and Lipoarabinomannan
- 7. Conjugation of synthetic oligosaccharides to proteins by squaric acid chemistry
- 8. Direct conjugation of bacterial polysaccharides to proteins by squaric acid chemistry
- 9. Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from Streptococcus equi for studies in cytokine production
- 10. O-Acetylation of Bacterial Cell Wall Polysaccharides
- 11. Designing glycosyltransferase expression constructs for improved purification, protein yield and crystallization
- 12. Utilization of fluorescently-tagged synthetic acceptor molecules for in vitro characterization of a dual-domain glycosyltransferase enzyme, KpsC, from Escherichia coli
- 13. Synthesis of Phenoxyundecyl Diphosphate Disaccharides for Studies of the Biosynthesis of O-Antigenic Polysaccharides in Enteric Bacteria
- 14. Characterization of arabinosyl transfer reactions in the biosynthesis of mycobacterial cell envelope (lipo)polysaccharides
- 15. Enzymatic synthesis of the repeating unit oligosaccharides of Escherichia coli O104
- 16. Sulfhydryl labelling as a tool to investigate the topology of membrane proteins involved in lipopolysaccharide biosynthesis
- 17. Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides
- 18. Glycosyltransferase Activity Assays Using Colorimetric Methods
- 19. In vitro characterization of a multidomain glycosyltransferase using fluorescently-tagged synthetic acceptors
- 20. Glycosyltransferase-coupled assays for 4-epimerase WbpP from Pseudomonas aeruginosa
- 21. Assaying fucosidase activity
- 22. In vitro synthesis of bioactive glycovariants of enterocin 96, an antimicrobial peptide from Enterococcus faecalis
- 23. High-Throughput Screening for Inhibitors of Wall Teichoic Acid Biosynthesis in Staphylococcus aureus.