T. cruzi Infection [E-Book] : Methods and Protocols / edited by Karina Andrea Gómez, Carlos Andrés Buscaglia.
Buscaglia, Carlos Andrés, (editor)
Gómez, Karina Andrea, (editor)
New York, NY : Humana Press, 2019
XVII, 398 pages 60 illustrations, 47 illustrations in color (online resource)
Methods in Molecular Biology ; 1955
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Table of Contents:
  • Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex Genome
  • Mining Trypanosoma cruzi Genome Sequences for Antigen Discovery and Vaccine Development
  • Transcriptome Studies in Trypanosoma cruzi using RNAseq
  • An Improved Approach to Trypanosoma cruzi Molecular Genotyping by Next-Generation Sequencing of the Mini-Exon Gene
  • Genome Editing by CRISPR/Cas9 in Trypanosoma cruzi
  • Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes
  • Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruzi
  • Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae
  • Homologous Expression of Glycosylphosphatidyl Inositol-Anchored Glycoproteins in Trypanosoma cruzi
  • Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi Trans-Sialidase
  • Exploiting Genetically Modified Dual-Reporter Strains to Monitor Experimental Trypanosoma cruzi Infections and Host: Parasite Interactions
  • Transmigration of Trypanosoma cruzi trypomastigotes through 3D Spheroids Mimicking Host Tissues
  • In Situ Detection of Dormant Trypanosoma cruzi Amastigotes using Bioluminescent-Fluorescent Reporters
  • Multi-Parametric Evaluation of Trypanosoma cruzi Infection Outcome in Animal Models
  • MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice
  • Genotyping of Trypanosoma cruzi from Clinical Samples by Multilocus Conventional PCR
  • Assessing Parasite Load in Chagas Disease Patients by Quantitative Multiplex Real-Time PCR
  • Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi trans-sialidase
  • Analytics of Functional Autoantibodies in Patients with Chagas' Disease
  • Apolipoprotein-A1 and Fibronectin Fragments as Markers of Cure for the Chagas Disease
  • Host Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers in Plasma
  • Purification of GlycosylphosphatidyLinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of a-Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas Disease
  • Cytometric Bead Array (CBA) for Measurement Cytokines Levels in Chagas Disease Patients
  • Generation of T. cruzi-specific Primary CD4+ T Cell Lines from Peripheral Blood Mononuclear Cells Isolated from Chagas Disease Patients
  • Trypanosma cruzi Specific T-Cell Responses to Monitor Treatment Efficacy in Chronic Chagas Disease
  • CD8+ T Cell Response to Trypanosoma cruzi Antigens during Chronic Chagas Disease
  • Fluorescence Proteomic Technology to Analyze Peripheral Blood Mononuclear Cells in Chronic Chagas Disease
  • Isolation and Phenotypic Characterization of Inflammatory Cells from Clinical Samples: Purification of Macrophages from Trypanosoma cruzi-Infected Hearts.