Neuroproteomics [E-Book] / edited by Ka Wan Li.
Li, Ka Wan, (editor)
2nd edition 2019.
New York, NY : Humana Press, 2019
XIII, 218 pages 55 illustrations, 37 illustrations in color (online resource)
englisch
9781493996629
10.1007/978-1-4939-9662-9
Neuromethods ; 146
Full Text
Table of Contents:
  • Neuroproteomics: The Methods
  • Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue
  • Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density Lattice
  • Laser Microdissection for Spatial Proteomics of Post Mortem Brain Tissue
  • High pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics
  • Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3)
  • Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip
  • Integrated Immunoprecipitation: Blue Native Gel Electrophoresis - Mass Spectrometry for the Identification of Protein Sub-Complexes
  • Analysis of Synaptic Protein-Protein Interaction by Crosslinking-Mass Spectrometry
  • TMT-MS3 Enabled Proteomic Quantification of Human IPSC Derived Neurons
  • Data Independent Acquisition (SWATH) Mass Spectrometry Analysis of Protein Content in Primary Neuronal Cultures
  • Quantitative Analysis of Mass Spectrometry-Based Proteomics Data
  • 2D-DIGE as a Tool in Neuroproteomics
  • Neuropeptidomics of the Mammalian Brain
  • MALDI Imaging Mass Spectrometry - Neurochemical Imaging of Proteins and Peptides
  • A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder.