ADAMTS Proteases [E-Book] : Methods and Protocols / edited by Suneel S. Apte.
Apte, Suneel S., (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
X, 299 pages 59 illustrations, 47 illustrations in color (online resource)
englisch
9781493996988
10.1007/978-1-4939-9698-8
Methods in Molecular Biology ; 2043
Full Text
Table of Contents:
  • ADAMTS Proteins: Concepts, Challenges, and Prospects
  • Inhibition of ADAMTS1 Expression by Lentiviral CRISPR/Cas9 Gene Editing Technology
  • Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins using Cell-Based Assays
  • Challenges and Solutions for Purification of ADAMTS Proteases: An Overview
  • Purification of Native or Recombinant ADAMTS2 and Procollagen I Cleavage Assay
  • ADAMTS7: Recombinant Protein Expression and Purification
  • Purification and Activity Determination of ADAMTS-4 and ADAMTS-5 and Their Domain Deleted Mutants
  • Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins
  • Assay for Reelin-Cleaving Activity of ADAMTS and Detection of Reelin and Its Fragments in the Brain
  • Chemical Modification of Proteoglycanases with Biotin
  • Analysis of Aggrecanase Activity using Neoepitope Antibodies
  • Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4
  • Purification of Recombinant ADAMTSL2
  • Expression Analysis by RNAscope In Situ Hybridization
  • Analysis of ADAMTS Effects on Cell Adhesion and Migration
  • Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin Microfibrils
  • Alizarin Red and Alcian Blue Preparations to Visualize the Skeleton
  • Biophysical Techniques to Analyze Elastic Tissue Extracellular Matrix Proteins Interacting with ADAMTS Proteins
  • Quantification of Extracellular Matrix Fiber Systems Related to ADAMTS Proteins
  • Visualization of Perineuronal Nets in Central Nervous System Tissue Sections
  • Visualization and Quantification of Pericellular Matrix
  • Quantitative Mass Spectrometry-Based Secretome Analysis as a Tool to Investigate Metalloprotease and TIMP Activity
  • Preparation and Application of a Decellularized Extracellular Matrix for Identification of ADAMTS Substrates
  • Mapping the N-Terminome in Tissue Biopsies by PCT-TAILS. .