Cryptosporidium [E-Book] : Methods and Protocols / edited by Jan R. Mead, Michael J. Arrowood.
Arrowood, Michael J., (editor)
Mead, Jan R., (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
XI, 407 pages 93 illustrations, 71 illustrations in color (online resource)
englisch
9781493997480
10.1007/978-1-4939-9748-0
Methods in Molecular Biology ; 2052
Full Text
Table of Contents:
  • Cryptosporidium Diagnostic Assays: Microscopy
  • Cryptosporidium Diagnostic Assays: Molecular Detection
  • Detection of Cryptosporidium Recovered From Large-Volume Water Samples Using Dead-End Ultrafiltration
  • Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation
  • Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay
  • Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii
  • Cryptosporidium Genotyping for Epidemiology Tracking
  • Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection
  • Preparation of Cryptosporidium DNA for Whole Genome Sequencing
  • Accessing Cryptosporidium Omic and Isolate Data Via CryptoDB.org
  • A Novel Method to Silence Genes in Cryptosporidium
  • Use of miRNAs to Study Host Cell-Parasite Interactions
  • Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9
  • Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology
  • Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics
  • Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water
  • High-Content Screening for Cryptosporidium Drug Discovery
  • High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR
  • In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds
  • In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial Monolayers
  • Two and Three Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium.