Herpes Simplex Virus [E-Book] : Methods and Protocols / edited by Russell J. Diefenbach, Cornel Fraefel.
Diefenbach, Russell J., (editor)
Fraefel, Cornel, (editor)
2nd edition 2020.
New York, NY : Humana Press, 2020
XIV, 457 pages 60 illustrations, 45 illustrations in color (online resource)
Methods in Molecular Biology ; 2060
Full Text
Table of Contents:
  • Tour de Herpes: Cycling through the Life and Biology of HSV-1
  • Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology
  • Herpes Simplex Virus Growth, Preparation, and Assay
  • Engineering HSV-1 Vectors for Gene Therapy
  • Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors
  • HSV-1 Amplicon Vectors as Genetic Vaccines
  • oHSV Genome Editing by Means of galK Recombineering
  • Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein
  • CRISPR/Cas9-Based Genome Editing of HSV
  • Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescent In Situ Hybridization (FISH)
  • Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for use in High-Throughput Sequencing
  • HSV Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo
  • Phenotypic and Genotypic Testing of HSV-1 and HSV-2 Resistance to Antivirals
  • Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation
  • Characterization of Extracellular HSV-1 Virions by Proteomics
  • Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry
  • Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells
  • Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot
  • BioID Combined with Mass Spectrometry to Study Herpesvirus Protein-Protein Interaction Networks
  • Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy
  • Transmission Immuno-Electron Microscopy of Herpes Simplex Virus-1 Infected Dorsal Root Ganglia Neurons Sectioned in Growth Plane
  • Multi-Fluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication
  • Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination
  • Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination
  • The Use of Microfluidic Neuronal Devices to Study the Anterograde Axonal Transport of Herpes Simplex Virus-1
  • A Model of In Vivo HSV-1 DNA Transport using Murine Retinal Ganglion Cells
  • The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines.