Purinergic Signaling [E-Book] : Methods and Protocols / edited by Pablo Pelegrín.
This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico m...
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Personal Name(s): | Pelegrín, Pablo, editor |
Edition: |
1st edition 2020. |
Imprint: |
New York, NY :
Humana Press,
2020
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Physical Description: |
XII, 360 pages 82 illustrations, 49 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781493997176 |
DOI: |
10.1007/978-1-4939-9717-6 |
Series Title: |
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Methods in Molecular Biology ;
2041 |
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Classification: |
- Introduction to Purinergic Signaling
- Knock-out and Knock-in Mouse Models to Study Purinergic Signaling
- Agonists and Antagonists for Purinergic Receptors
- Homology Modelling of P2X Receptors
- Using RNA Interference for Purinoceptor Knock Down in vivo
- Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole Mount in situ hybridization in Xenopus Embryos
- Histochemical Approach for Simultaneous Detection of Ecto-nucleotidase and Alkaline Phosphatase Activities in Tissues
- Flow Cytometry of Membrane Purinoreceptors
- Studying Purinoceptor Cell-surface Expression by Protein Biotinylation
- Multimeric Ionotropic Purinoceptor Detection by Protein Cross-linking
- Multimeric Purinoceptor Detection by Bioluminiscence Resonance Energy Transfer
- Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy
- Detection of Extracellular ATP in the Tumor Microenvironment, using the pmeLUC Biosensor
- Using Amperometric, Enzyme-Based Biosensors for Performing Longitudinal Measurements of Extracellular adenosine 5-triphosphate in the Mouse
- Fluorescent Labeling and Quantification of Vesicular ATP Release using Live Cell Imaging
- Using FRET-based Fluorescent Sensors to Monitor Cytosolic and Membrane-proximal Extracellular ATP levels
- ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader
- P2X Electrophysiology and Surface Trafficking in Xenopus oocytes
- Heterologous Expression and Patch-clamp Recording of P2X Receptors in HEK293 Cells
- Recording P2X Receptors Using Whole Cell Patch Clamp from Native Monocytes and Macrophages
- Automated Planar Patch-clamp Recording of P2X Receptors
- Controlling Engineered P2X Receptors with Light
- Intracellular Calcium Recording after Purinoceptor Activation Using a Video-microscopy Equipment
- Assays to Measure Purinoceptor Pore Dilation
- Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization
- Measuring Leukocyte Migration to Nucleotides
- Assessment of Cell Adhesion after Purinoceptor Activation.