Purinergic Signaling [E-Book] : Methods and Protocols / edited by Pablo Pelegrín.
Pelegrín, Pablo, (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
XII, 360 pages 82 illustrations, 49 illustrations in color (online resource)
Methods in Molecular Biology ; 2041
Full Text
Table of Contents:
  • Introduction to Purinergic Signaling
  • Knock-out and Knock-in Mouse Models to Study Purinergic Signaling
  • Agonists and Antagonists for Purinergic Receptors
  • Homology Modelling of P2X Receptors
  • Using RNA Interference for Purinoceptor Knock Down in vivo
  • Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole Mount in situ hybridization in Xenopus Embryos
  • Histochemical Approach for Simultaneous Detection of Ecto-nucleotidase and Alkaline Phosphatase Activities in Tissues
  • Flow Cytometry of Membrane Purinoreceptors
  • Studying Purinoceptor Cell-surface Expression by Protein Biotinylation
  • Multimeric Ionotropic Purinoceptor Detection by Protein Cross-linking
  • Multimeric Purinoceptor Detection by Bioluminiscence Resonance Energy Transfer
  • Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy
  • Detection of Extracellular ATP in the Tumor Microenvironment, using the pmeLUC Biosensor
  • Using Amperometric, Enzyme-Based Biosensors for Performing Longitudinal Measurements of Extracellular adenosine 5-triphosphate in the Mouse
  • Fluorescent Labeling and Quantification of Vesicular ATP Release using Live Cell Imaging
  • Using FRET-based Fluorescent Sensors to Monitor Cytosolic and Membrane-proximal Extracellular ATP levels
  • ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader
  • P2X Electrophysiology and Surface Trafficking in Xenopus oocytes
  • Heterologous Expression and Patch-clamp Recording of P2X Receptors in HEK293 Cells
  • Recording P2X Receptors Using Whole Cell Patch Clamp from Native Monocytes and Macrophages
  • Automated Planar Patch-clamp Recording of P2X Receptors
  • Controlling Engineered P2X Receptors with Light
  • Intracellular Calcium Recording after Purinoceptor Activation Using a Video-microscopy Equipment
  • Assays to Measure Purinoceptor Pore Dilation
  • Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization
  • Measuring Leukocyte Migration to Nucleotides
  • Assessment of Cell Adhesion after Purinoceptor Activation.