Antibody-Drug Conjugates [E-Book] : Methods and Protocols / edited by L. Nathan Tumey.
Tumey, L. Nathan, (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
XI, 373 pages 138 illustrations, 109 illustrations in color (online resource)
Methods in Molecular Biology ; 2078
Full Text
Table of Contents:
  • An Overview of the Current ADC Discovery Landscape
  • Pushing the Envelope: Advancement of ADCs Outside of Oncology
  • Conjugations to Endogenous Cysteine Residues
  • Site-Specific Conjugation to Cys-Engineered THIOMAB Antibodies
  • Transglutaminase-Mediated Conjugations
  • Click Chemistry Conjugations
  • Utilizing Solid-Phase to Enable High-Throughput, Site-Specific Conjugation and Dual Labeled Antibody and Fab Conjugates
  • Bridged Cysteine Conjugations
  • Antibody Conjugations via Glycosyl Remodeling
  • ADC Analysis by Hydrophobic Interaction Chromatography
  • Two-Dimensional Liquid Chromatography Coupled to High Resolution Mass Spectrometry for the Analysis of ADCs
  • Drug Loading and Distribution of ADCs after Reduction or IdeS Digestion and Reduction
  • Analysis of ADCs by Native Mass Spectrometry
  • High Resolution Characterization of ADCs by Orbitrap LCMS
  • Conjugation Site Analysis by MS/MS Protein Sequencing
  • Conjugation Site Analysis of Lysine-Conjugated ADCs
  • Characterization of ADCs by Capillary Electrophoresis
  • Characterization of the Primary Structure of Cysteine-Linked Antibody-Drug Conjugates using Capillary Electrophoresis with Mass Spectrometry
  • Purification of ADCs by Hydrophobic Interaction Chromatography
  • Detection and Removal of Small-Molecule and Endotoxin Contaminants in ADC Preparations
  • Physical Stability Studies of Antibody Drug Conjugates (ADCs) Under Stressed Conditions
  • Biophysical Methods for Characterization of Antibody Drug Conjugates
  • Determination of ADC Cytotoxicity in Immortalized Human Cell Lines
  • LC/MS Methods for Studying Lysosomal ADC Catabolism
  • Assessing ADC Plasma Stability by LC/MS Methods
  • Determination of ADC Concentration by Ligand-Binding Assays.