Transgenic Mouse [E-Book] : Methods and Protocols / edited by Melissa A. Larson.
Larson, Melissa A., (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
X, 236 pages 45 illustrations, 27 illustrations in color (online resource)
Methods in Molecular Biology ; 2066
Full Text
Table of Contents:
  • The History of Transgenesis
  • Pronuclear Microinjection of One-Cell Embryos
  • Integrase-Mediated Targeted Transgenics through Pronuclear Microinjection
  • In Vivo Validation of CRISPR Reagents in Pre-Implantation Mouse Embryos
  • Targeted Mutations in the Mouse via Embryonic Stem Cells
  • Blastocyst Microinjection with Embryonic Stem Cells
  • Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos
  • Embryo Transfer Surgery
  • Non-Surgical Embryo Transfer Protocol for use with the NSET Device
  • Nuclear Transfer and Cloning
  • Sperm-Mediated Genetic Modifications
  • Genotyping Genetically Modified (GM) Mice
  • Nomenclature: Naming Your Gene-Modified Mouse
  • Breeding Strategies for Genetically Modified Mice
  • Strategies for Behaviorally Phenotyping the Transgenic Mouse
  • Cryobanking and Recovery of Genetically Modified Mice
  • Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques
  • Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells using Sendai Virus.