Quantitative Real-Time PCR [E-Book] : Methods and Protocols / edited by Roberto Biassoni, Alessandro Raso.
Biassoni, Roberto, (editor)
Raso, Alessandro, (editor)
2nd edition 2020.
New York, NY : Humana Press, 2020
XIII, 235 pages 70 illustrations, 55 illustrations in color (online resource)
Methods in Molecular Biology ; 2065
Full Text
Table of Contents:
  • A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers
  • Parameters for Successful PCR Primer Design
  • MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing
  • Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation
  • A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR
  • Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR)
  • Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR)
  • Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine
  • A General Protocol for Accurate Gene Expression Analysis in Plants
  • Gene Expression Analysis in Bacteria by RT-qPCR
  • Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR
  • Molecular Monitoring of Chronic Myeloid Leukemia
  • Normalization in Human Gliomas Tissue
  • qPCR Applications for the Determination of the Biological Age
  • QuantStudio 12K Flex OpenArray┬« System as a Tool for High-Throughput Genotyping and Gene Expression Analysis
  • Digital PCR and the QuantStudio 3D Digital PCR System.